Detailed information of TA system TA194910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5245960..5246600
• Replicon: chromosome
• Accession: NZ_CP070982
• Organism: Pseudomonas entomophila strain Small

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JYG34_RS23010
• Protein ID: WP_213658481.1
• Coordinates: 5246190..5246600 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q1I4A9
• Locus tag: JYG34_RS23005
• Protein ID: WP_011535889.1
• Coordinates: 5245960..5246190 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYG34_RS22985 (JYG34_22985)	5241284..5242609	-	1326	WP_213658477.1	precorrin-3B synthase	-
JYG34_RS22990 (JYG34_22990)	5242853..5244064	+	1212	WP_213658478.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
JYG34_RS22995 (JYG34_22995)	5244057..5245151	+	1095	WP_213658479.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
JYG34_RS23000 (JYG34_23000)	5245148..5245876	+	729	WP_213658480.1	cobalt-precorrin-6A reductase	-
JYG34_RS23005 (JYG34_23005)	5245960..5246190	+	231	WP_011535889.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JYG34_RS23010 (JYG34_23010)	5246190..5246600	+	411	WP_213658481.1	tRNA(fMet)-specific endonuclease VapC	Toxin
JYG34_RS23015 (JYG34_23015)	5246673..5247110	+	438	WP_011535891.1	NfeD family protein	-
JYG34_RS23020 (JYG34_23020)	5247138..5247992	+	855	WP_011535892.1	SPFH domain-containing protein	-
JYG34_RS23025 (JYG34_23025)	5248140..5248529	+	390	WP_213658482.1	DUF2946 domain-containing protein	-
JYG34_RS23030 (JYG34_23030)	5248577..5249059	+	483	WP_213658483.1	copper chaperone PCu(A)C	-
JYG34_RS23035 (JYG34_23035)	5249084..5249515	+	432	WP_213658484.1	DUF2946 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15286.66 Da
• Isoelectric Point: 9.3867

>T194910 WP_213658481.1 NZ_CP070982:5246190-5246600 [Pseudomonas entomophila]
MLRYMLDTNICIFTIKNKPQVVREAFNRYHGQLAISTVTLMELIYGAEKSAAPERNLAIVEGFAARLDVLDYDIQGAAHT
AQLRAELAKAGTPIGPYDRMIAGHARARGLTLVTNNLREFQRVPGLRVEDWVTYPR

Nucleotide

• Length: 411 bp

>T194910 NZ_CP094178:583040-583306 [Helicobacter pylori]
ATGCTGAAAATTAAGCACCATATCCTTTACAAAAAAGATTTTAAAAAACTTATTAAAAACGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAACCTTAAGAAAAAAAGAACCACTAGACCCACAATTTAAAGATCATGCTTTAAAAGGCAATTGGA
AGCCTTTTAGGGAATGCCACATCAAAGCTGATATTTTACTTGTGTATTTAGTGAAAGGTGATGAGCTTATTTTGTTAAGA
CTAGGCAGTCATAGCGAGCTGTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8443.36 Da
• Isoelectric Point: 4.2821

>AT194910 WP_011535889.1 NZ_CP070982:5245960-5246190 [Pseudomonas entomophila]
MEQGAVFKSNRSQAIRMPKALALPEDVTRVDIVAVGRSRIISPAGESWDSWFEGDDVSADFMASREQPADQEREGF

Nucleotide

• Length: 231 bp

>AT194910 NZ_CP094178:582682-583053 [Helicobacter pylori]
ATGCCTAACTCTTCTACCAAAAAAGACTACACACAATACAGCGAAAAACAGCTTTTTAACTTAATCAACCAATTAGAGCA
AAAAATCAAAAAGATGCAAAATGATAGAGTTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCATTAAATGAACTCTTGCAAAAAATCAGCCAAGCTTTTGACGATAAAAGAGATTGTTGTTTG
GGGCGCAAGATCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGTTAAATGGCGAAAAATTAGAAACTATTGAAGA
CCTTTCTGCATGGGCAAATGAAGTGAAAAAGGAAGTGAATGCTGAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A2V4HYG6