Detailed information of TA system TA194876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 531169..531805
• Replicon: chromosome
• Accession: NZ_CP070976
• Organism: Bacillus halotolerans strain MBH1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: JYG31_RS02655
• Protein ID: WP_003156187.1
• Coordinates: 531455..531805 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: JYG31_RS02650
• Protein ID: WP_003225183.1
• Coordinates: 531169..531450 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYG31_RS02630 (527524)	527524..528123	-	600	WP_024120294.1	rhomboid family intramembrane serine protease	-
JYG31_RS02635 (528218)	528218..528583	+	366	WP_024120295.1	holo-ACP synthase	-
JYG31_RS02640 (528749)	528749..529765	+	1017	WP_082687822.1	outer membrane lipoprotein carrier protein LolA	-
JYG31_RS02645 (529882)	529882..531051	+	1170	WP_103671960.1	alanine racemase	-
JYG31_RS02650 (531169)	531169..531450	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
JYG31_RS02655 (531455)	531455..531805	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
JYG31_RS02660 (531920)	531920..532744	+	825	WP_081638235.1	RsbT co-antagonist protein RsbRA	-
JYG31_RS02665 (532749)	532749..533114	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
JYG31_RS02670 (533118)	533118..533519	+	402	WP_024120299.1	serine/threonine-protein kinase RsbT	-
JYG31_RS02675 (533531)	533531..534538	+	1008	WP_024120300.1	phosphoserine phosphatase RsbU	-
JYG31_RS02680 (534599)	534599..534928	+	330	WP_024120301.1	anti-sigma factor antagonist RsbV	-
JYG31_RS02685 (534925)	534925..535407	+	483	WP_024120302.1	anti-sigma B factor RsbW	-
JYG31_RS02690 (535373)	535373..536161	+	789	WP_024120303.1	RNA polymerase sigma factor SigB	-
JYG31_RS02695 (536161)	536161..536760	+	600	WP_024120304.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T194876 WP_003156187.1 NZ_CP070976:531455-531805 [Bacillus halotolerans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T194876 NZ_CP094144:484231-484512 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTAAAGAAAGCGA
TGTTACGAGCGTTGTTGATTGTCTCAAAGAGCAAAAACCACTCCAACAAAAACATTGCGACCATGCCTTGAGTGGCAATC
TTAAAGGGTTGATGGAATGCCATGTTAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT194876 WP_003225183.1 NZ_CP070976:531169-531450 [Bacillus halotolerans]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT194876 NZ_CP094144:483924-484238 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTATTCGCAATATAATGAAAAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGTGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGATTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32