Detailed information of TA system TA194853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1115173..1115778
• Replicon: chromosome
• Accession: NZ_CP070969
• Organism: Paenibacillus sp. TB2019

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0W1B4L6
• Locus tag: JRJ22_RS04815
• Protein ID: WP_042124727.1
• Coordinates: 1115428..1115778 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JRJ22_RS04810
• Protein ID: WP_187388800.1
• Coordinates: 1115173..1115424 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRJ22_RS04795	1111426..1112268	+	843	WP_206103475.1	hypothetical protein	-
JRJ22_RS04800	1112443..1113525	+	1083	WP_206103476.1	DUF4367 domain-containing protein	-
JRJ22_RS04805	1113750..1114931	+	1182	WP_206103477.1	alanine racemase	-
JRJ22_RS04810	1115173..1115424	+	252	WP_187388800.1	ribbon-helix-helix protein, CopG family	Antitoxin
JRJ22_RS04815	1115428..1115778	+	351	WP_042124727.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JRJ22_RS04820	1115995..1116552	+	558	WP_206103478.1	TetR family transcriptional regulator	-
JRJ22_RS04825	1116757..1117962	+	1206	WP_206103479.1	MFS transporter	-
JRJ22_RS04830	1118132..1120312	+	2181	WP_206104978.1	helix-hairpin-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12764.68 Da
• Isoelectric Point: 5.1171

>T194853 WP_042124727.1 NZ_CP070969:1115428-1115778 [Paenibacillus sp. TB2019]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDETMKKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T194853 NZ_CP094130:c101246-100980 [Helicobacter pylori]
GTGTTGAAACTCAACCTTAAAAAATCTTTTCAAAAAGATTTTGATAAATTACTTTTGAATGGGTTTGATGATAGCGTTTT
GAATAAGGTCATTCTGTCTTTGAGAAAAAAAGAACCGCTAGACCCACAATTTCAAGATCATGCCTTGAAAGGAAAGTGGA
AACCTTTTAGGGAATGCCACATCAAAGCAGATATTTTGCTTGTGTATTTAGTGAAAGGTGATGAGCTTATTTTAGTAAGA
CTAGGCAGTCATAGCGAGCTGTTTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9443.87 Da
• Isoelectric Point: 5.1186

>AT194853 WP_187388800.1 NZ_CP070969:1115173-1115424 [Paenibacillus sp. TB2019]
MISLPDHLLQEVDGIAQLENSNRSELIRQAMKLYLTERKKRTIRESMQRGYMEMAKINLTMASEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT194853 NZ_CP094130:c101604-101227 [Helicobacter pylori]
ATGCCTAACTCTTCTGCCAAAAAAGATTACACACGATACAGCGAAAAACAGCTTTTTAACTTAATCAACCAGTTAGAGCA
AAAAATCAAAAAGATGCAAAATGATAGAGCTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCATTAAATGAACTCTTGCAAAAAATCAGCCAAGCTTTTGATAATAAAAGAGATTGTTGTTTT
GGGCATGAAACCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGCTTAACAAAGAAACCGATTTGATTGTTGAGGA
TTTTTCTAGTTATAGCGATGAAAGAAAAAGGGCGTTAGGTGTTGAAACTCAACCTTAA

Structures

Toxin

• AlphaFold DB: A0A0W1B4L6