Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 95123..95766 Replicon plasmid pMG101_B
Accession NZ_CP070963
Organism Escherichia coli strain K-12 J53

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag JTR57_RS22970 Protein ID WP_001034044.1
Coordinates 95350..95766 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag JTR57_RS22965 Protein ID WP_001261286.1
Coordinates 95123..95353 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTR57_RS22950 (90260) 90260..90490 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
JTR57_RS22955 (90487) 90487..90903 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
JTR57_RS22960 (90948) 90948..94742 - 3795 WP_001144732.1 hypothetical protein -
JTR57_RS22965 (95123) 95123..95353 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JTR57_RS22970 (95350) 95350..95766 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
JTR57_RS22975 (95841) 95841..97406 + 1566 WP_001128474.1 AAA family ATPase -
JTR57_RS22980 (97391) 97391..98413 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / ant(3'')-Ia / blaOXA-1 / sitABCD / tet(B) / catA1 iutA / iucD / iucC / iucB / iucA 1..154497 154497
- flank IS/Tn - - 98667..99170 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T194838 WP_001034044.1 NZ_CP070963:95350-95766 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T194838 NZ_CP094113:603324-603590 [Helicobacter pylori]
GTGTTGAAACTCAATCTTAAAAAATCTTTTCAAAAAGATTTTGATAAATTACTTTTGAATGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAACCTTAAGAAAGAAAGAACCGCTAGATCCACAATTTAAAGATCATGCTTTAAAAGGCAATTGGA
AGCCTTTTAGGGAATGCCACATTAAACCTGATATTTTGCTTGTGTATTTAGTGAAAGATGATGAGCTTATTTTAGTAAGA
CTAGGCAGTCATAGCGAGCTGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT194838 WP_001261286.1 NZ_CP070963:95123-95353 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT194838 NZ_CP094113:602966-603343 [Helicobacter pylori]
ATGCCTAACACCACCGCCAAAAAAGACTACACACAATACAGCGAAAAACAGCTTTTTAACTTAATCAACCAATTAGAGCA
AAAGATCAAAAAGATGCAAAATGATAGAGCTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCGTTAAATGAACTCTTGCAAAAAATCAGCCAAGCTTTTGGTGATAAAAGAGATTGTTGTTTG
GGGCGCAAGATCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGCTTAACAAAGAAACCGATTTGATTGTTGAGGA
TTTTTCTAGTTATAGCGATGAAAGAAAAAGGGCGTTAGGTGTTGAAACTCAATCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References