Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 90260..90903 Replicon plasmid pMG101_B
Accession NZ_CP070963
Organism Escherichia coli strain K-12 J53

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag JTR57_RS22955 Protein ID WP_001034046.1
Coordinates 90487..90903 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag JTR57_RS22950 Protein ID WP_001261278.1
Coordinates 90260..90490 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTR57_RS22920 (85771) 85771..86076 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
JTR57_RS22925 (86078) 86078..86296 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
JTR57_RS22930 (86863) 86863..87375 + 513 WP_000151784.1 hypothetical protein -
JTR57_RS22935 (87409) 87409..88542 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
JTR57_RS22940 (88709) 88709..89482 - 774 WP_000905949.1 hypothetical protein -
JTR57_RS22945 (89495) 89495..89995 - 501 WP_000528931.1 HEPN family nuclease -
JTR57_RS22950 (90260) 90260..90490 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JTR57_RS22955 (90487) 90487..90903 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
JTR57_RS22960 (90948) 90948..94742 - 3795 WP_001144732.1 hypothetical protein -
JTR57_RS22965 (95123) 95123..95353 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
JTR57_RS22970 (95350) 95350..95766 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / ant(3'')-Ia / blaOXA-1 / sitABCD / tet(B) / catA1 iutA / iucD / iucC / iucB / iucA 1..154497 154497


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T194837 WP_001034046.1 NZ_CP070963:90487-90903 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T194837 NZ_CP094113:500153-500434 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTAAAGAAAGCGA
TGTTACAAGCGTTGTTGATTGTCTCAAAGAGCAAAAACCGCTCCAACAAAAGTATTGCGACCATGCCTTAAGTGGCAATC
TTAAAGGGTTGAGAGAGTGTCATGTCAAGCCAAACTTGCTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT194837 WP_001261278.1 NZ_CP070963:90260-90490 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT194837 NZ_CP094113:499846-500160 [Helicobacter pylori]
ATGTCAGATGTTATTACAGATTATTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References