Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 85771..86296 | Replicon | plasmid pMG101_B |
| Accession | NZ_CP070963 | ||
| Organism | Escherichia coli strain K-12 J53 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | V0SSI5 |
| Locus tag | JTR57_RS22920 | Protein ID | WP_001159868.1 |
| Coordinates | 85771..86076 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | S1PPD8 |
| Locus tag | JTR57_RS22925 | Protein ID | WP_000813634.1 |
| Coordinates | 86078..86296 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JTR57_RS22905 (81681) | 81681..82847 | - | 1167 | WP_000772446.1 | plasmid-partitioning protein SopA | - |
| JTR57_RS22910 (83435) | 83435..84190 | - | 756 | WP_000852146.1 | replication initiation protein RepE | - |
| JTR57_RS22915 (84964) | 84964..85770 | - | 807 | WP_000016982.1 | site-specific integrase | - |
| JTR57_RS22920 (85771) | 85771..86076 | - | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| JTR57_RS22925 (86078) | 86078..86296 | - | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| JTR57_RS22930 (86863) | 86863..87375 | + | 513 | WP_000151784.1 | hypothetical protein | - |
| JTR57_RS22935 (87409) | 87409..88542 | - | 1134 | WP_000545987.1 | DUF3800 domain-containing protein | - |
| JTR57_RS22940 (88709) | 88709..89482 | - | 774 | WP_000905949.1 | hypothetical protein | - |
| JTR57_RS22945 (89495) | 89495..89995 | - | 501 | WP_000528931.1 | HEPN family nuclease | - |
| JTR57_RS22950 (90260) | 90260..90490 | + | 231 | WP_001261278.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| JTR57_RS22955 (90487) | 90487..90903 | + | 417 | WP_001034046.1 | type II toxin-antitoxin system VapC family toxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | sul1 / qacE / ant(3'')-Ia / blaOXA-1 / sitABCD / tet(B) / catA1 | iutA / iucD / iucC / iucB / iucA | 1..154497 | 154497 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11706.51 Da Isoelectric Point: 6.4674
>T194836 WP_001159868.1 NZ_CP070963:c86076-85771 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T194836 NZ_CP094111:603316-603582 [Helicobacter pylori]
GTGTTGAAACTCAATCTTAAAAAATCTTTTCAAAAAGATTTTGATAAATTACTTTTGAATGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAACCTTAAGAAAGAAAGAACCGCTAGATCCACAATTTAAAGATCATGCTTTAAAAGGCAATTGGA
AGCCTTTTAGGGAATGCCACATTAAACCTGATATTTTGCTTGTGTATTTAGTGAAAGATGATGAGCTTATTTTAGTAAGA
CTAGGCAGTCATAGCGAGCTGTTTTAA
GTGTTGAAACTCAATCTTAAAAAATCTTTTCAAAAAGATTTTGATAAATTACTTTTGAATGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAACCTTAAGAAAGAAAGAACCGCTAGATCCACAATTTAAAGATCATGCTTTAAAAGGCAATTGGA
AGCCTTTTAGGGAATGCCACATTAAACCTGATATTTTGCTTGTGTATTTAGTGAAAGATGATGAGCTTATTTTAGTAAGA
CTAGGCAGTCATAGCGAGCTGTTTTAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8372.34 Da Isoelectric Point: 4.7405
>AT194836 WP_000813634.1 NZ_CP070963:c86296-86078 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT194836 NZ_CP094111:602958-603335 [Helicobacter pylori]
ATGCCTAACACCACCGCCAAAAAAGACTACACACAATACAGCGAAAAACAGCTTTTTAACTTAATCAACCAATTAGAGCA
AAAGATCAAAAAGATGCAAAATGATAGAGCTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCGTTAAATGAACTCTTGCAAAAAATCAGCCAAGCTTTTGGTGATAAAAGAGATTGTTGTTTG
GGGCGCAAGATCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGCTTAACAAAGAAACCGATTTGATTGTTGAGGA
TTTTTCTAGTTATAGCGATGAAAGAAAAAGGGCGTTAGGTGTTGAAACTCAATCTTAA
ATGCCTAACACCACCGCCAAAAAAGACTACACACAATACAGCGAAAAACAGCTTTTTAACTTAATCAACCAATTAGAGCA
AAAGATCAAAAAGATGCAAAATGATAGAGCTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCGTTAAATGAACTCTTGCAAAAAATCAGCCAAGCTTTTGGTGATAAAAGAGATTGTTGTTTG
GGGCGCAAGATCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGCTTAACAAAGAAACCGATTTGATTGTTGAGGA
TTTTTCTAGTTATAGCGATGAAAGAAAAAGGGCGTTAGGTGTTGAAACTCAATCTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|