Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3829777..3830274 Replicon chromosome
Accession NZ_CP070962
Organism Escherichia coli strain K-12 J53

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JTR57_RS18755 Protein ID WP_001173343.1
Coordinates 3829777..3829950 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JTR57_RS18760 Protein ID WP_000398619.1
Coordinates 3829978..3830274 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTR57_RS18745 (3826447) 3826447..3827904 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
JTR57_RS18750 (3828141) 3828141..3829658 + 1518 WP_001295074.1 lysine--tRNA ligase -
JTR57_RS18755 (3829777) 3829777..3829950 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JTR57_RS18760 (3829978) 3829978..3830274 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JTR57_RS18765 (3830501) 3830501..3830773 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
JTR57_RS18770 (3830785) 3830785..3831015 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
JTR57_RS18775 (3831196) 3831196..3832827 + 1632 WP_001216477.1 sensor histidine kinase -
JTR57_RS18780 (3832824) 3832824..3833543 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T194826 WP_001173343.1 NZ_CP070962:c3829950-3829777 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T194826 NZ_CP094101:c1428976-1428695 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCTGGGCGTATTACAGAAAGCGA
TGTTGTAAGCGTTGTTGATTGTCTCAAAGAGCAAAAACCGCTCCAACAAAAGTATTGCGACCATGCCTTAAGTGGCAATC
TTAAAGGGTTGAGAGAATGCCATGTCAAGCCAAACTTGCTGTTGATTTATGAAGTCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT194826 WP_000398619.1 NZ_CP070962:c3830274-3829978 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT194826 NZ_CP094101:c1429283-1428969 [Helicobacter pylori]
ATGTCAAATATTACTACAGATTACTCACAATACAATGAAAAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References