Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3071258..3071876 Replicon chromosome
Accession NZ_CP070962
Organism Escherichia coli strain K-12 J53

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JTR57_RS15065 Protein ID WP_001291435.1
Coordinates 3071658..3071876 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JTR57_RS15060 Protein ID WP_000344800.1
Coordinates 3071258..3071632 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTR57_RS15050 (3066347) 3066347..3067540 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JTR57_RS15055 (3067563) 3067563..3070712 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JTR57_RS15060 (3071258) 3071258..3071632 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JTR57_RS15065 (3071658) 3071658..3071876 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JTR57_RS15070 (3072048) 3072048..3072599 + 552 WP_000102564.1 maltose O-acetyltransferase -
JTR57_RS15075 (3072715) 3072715..3073185 + 471 WP_000136192.1 YlaC family protein -
JTR57_RS15080 (3073349) 3073349..3074899 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JTR57_RS15085 (3074941) 3074941..3075294 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JTR57_RS15095 (3075673) 3075673..3075984 + 312 WP_000409911.1 MGMT family protein -
JTR57_RS15100 (3076015) 3076015..3076587 - 573 WP_000779842.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T194819 WP_001291435.1 NZ_CP070962:3071658-3071876 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T194819 NZ_CP094094:c1069100-1068891 [Helicobacter pylori]
ATGCTGATGATTGAAACCAATAAAAAATTTGATAAGGATCTTAAAATTCTTGTTAAAAACGGGTTTGATTTAAAGCTTTT
ATATAAAGTGGTTGAAAATTTAGCCAAAGAGCAACCCCTAGCTCCCAAATACAAAGACCACCCTCTCAAAGGCGCTTTAA
AAGATTTTAGGGAATGCCACTTAAAAAACAAGAAAACACCCTCTTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT194819 WP_000344800.1 NZ_CP070962:3071258-3071632 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT194819 NZ_CP094094:c1069398-1069087 [Helicobacter pylori]
ATGCCTAATTCTCCCACCAAAAAAGACTACACACAATACAGCGAAAAACAGCTTTTTAACTTTTTAAATTCTATTAAGAC
CAAACAAAAAAGAGCGTTAGAAAAATTGAAAGAAATCCAAGCTCAAAAACAAAGAATCAAAAAAGCGCTCCAATTTAAAG
CGCTCCATTTAACCGAAAACGGATACACCATAGAAGAAGAGCGAGAAATCTTAGCAAGGGCTAAAGACACCAAGAACCAC
CTTTGTTTTAAAAGCGTAGAGGATTTTAAAAAACATTGTGAAAACTTAAGATTTAAAAATGCTGATGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References