Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2058076..2058714 Replicon chromosome
Accession NZ_CP070962
Organism Escherichia coli strain K-12 J53

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JTR57_RS10055 Protein ID WP_000813794.1
Coordinates 2058538..2058714 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JTR57_RS10050 Protein ID WP_001270286.1
Coordinates 2058076..2058492 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTR57_RS10030 (2053228) 2053228..2054169 - 942 WP_001251304.1 ABC transporter permease -
JTR57_RS10035 (2054170) 2054170..2055183 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
JTR57_RS10040 (2055201) 2055201..2056346 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
JTR57_RS10045 (2056591) 2056591..2057997 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
JTR57_RS10050 (2058076) 2058076..2058492 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JTR57_RS10055 (2058538) 2058538..2058714 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JTR57_RS10060 (2058936) 2058936..2059166 + 231 WP_000494244.1 YncJ family protein -
JTR57_RS10065 (2059258) 2059258..2061219 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JTR57_RS10070 (2061292) 2061292..2061828 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
JTR57_RS10075 (2061920) 2061920..2063095 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T194818 WP_000813794.1 NZ_CP070962:c2058714-2058538 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T194818 NZ_CP094094:c57218-56937 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCTGGGCGTATTAAAGAAAGCGA
TGTTACGAGCGTTGTTGATTGTCTCAAAGAGCAAAAACCACTCCAACAAAAATATTGCGACCATGCCTTGAGTGGCAATC
TTAAAGGGTTGAGGGAATGCCATGTCAAGCCAAACTTGCTGTTGATTTATGAAGTCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCACAGCGAGCTATTTAAAAAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT194818 WP_001270286.1 NZ_CP070962:c2058492-2058076 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT194818 NZ_CP094094:c57525-57211 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAGAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATG
GCTTAAGGCAGAAAGGGATAAAAACAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References