Detailed information of TA system TA194818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2058076..2058714
• Replicon: chromosome
• Accession: NZ_CP070962
• Organism: Escherichia coli strain K-12 J53

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JTR57_RS10055
• Protein ID: WP_000813794.1
• Coordinates: 2058538..2058714 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JTR57_RS10050
• Protein ID: WP_001270286.1
• Coordinates: 2058076..2058492 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTR57_RS10030 (2053228)	2053228..2054169	-	942	WP_001251304.1	ABC transporter permease	-
JTR57_RS10035 (2054170)	2054170..2055183	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
JTR57_RS10040 (2055201)	2055201..2056346	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
JTR57_RS10045 (2056591)	2056591..2057997	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
JTR57_RS10050 (2058076)	2058076..2058492	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JTR57_RS10055 (2058538)	2058538..2058714	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JTR57_RS10060 (2058936)	2058936..2059166	+	231	WP_000494244.1	YncJ family protein	-
JTR57_RS10065 (2059258)	2059258..2061219	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JTR57_RS10070 (2061292)	2061292..2061828	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
JTR57_RS10075 (2061920)	2061920..2063095	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T194818 WP_000813794.1 NZ_CP070962:c2058714-2058538 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T194818 NZ_CP094094:c57218-56937 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCTGGGCGTATTAAAGAAAGCGA
TGTTACGAGCGTTGTTGATTGTCTCAAAGAGCAAAAACCACTCCAACAAAAATATTGCGACCATGCCTTGAGTGGCAATC
TTAAAGGGTTGAGGGAATGCCATGTCAAGCCAAACTTGCTGTTGATTTATGAAGTCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCACAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT194818 WP_001270286.1 NZ_CP070962:c2058492-2058076 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT194818 NZ_CP094094:c57525-57211 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAGAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATG
GCTTAAGGCAGAAAGGGATAAAAACAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Toxin

• PDB: 6HPB