Detailed information of TA system TA194803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 648354..648937
• Replicon: chromosome
• Accession: NZ_CP070962
• Organism: Escherichia coli strain K-12 J53

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S1EZP4
• Locus tag: JTR57_RS03160
• Protein ID: WP_000254738.1
• Coordinates: 648602..648937 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: JTR57_RS03155
• Protein ID: WP_000581937.1
• Coordinates: 648354..648602 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTR57_RS03145 (644693)	644693..645994	+	1302	WP_000046812.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
JTR57_RS03150 (646042)	646042..648276	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
JTR57_RS03155 (648354)	648354..648602	+	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JTR57_RS03160 (648602)	648602..648937	+	336	WP_000254738.1	endoribonuclease MazF	Toxin
JTR57_RS03165 (649008)	649008..649799	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
JTR57_RS03170 (650027)	650027..651664	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
JTR57_RS03175 (651752)	651752..653050	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T194803 WP_000254738.1 NZ_CP070962:648602-648937 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T194803 NZ_CP094082:18302-19084 [Helicobacter pylori]
ATGGAAACTAAAGAAGAAGATAAAGATAAAAAGCTAGAGAAAATTATTGTATTGCTGTGCGAAGAGGGGGATTTGTCCAG
CCAAAAAGATCAAATCATCAAAGATCTCAAAGAAATCTATAAAGGAGAATACAAGCATAAATATTCAAAAATCACAACTA
TTATTTTAAATTCCACAAGGGATAAAGAGCAAGCCTTTATGATGCTCACACAAAATATAAAGACACTCAAAGAGATTCAA
GATAATAAAGAAGTTGAAAGCATTAAACCAAAACTAGAAAAGCTTTATGATCACATGAATTTAGAGTGTATTAGACTGCA
AGATTTTGATGAGAAAATGAGTAGAGTTAAAAATGTTTCTATAAAGTTAGAAGATGAACTAAATAAAAACTATAAAAAAT
TAAGCGAAGAATTGAATAAGCAACAAACACAATATATAACAATTTTAGGTATTTTTGCCTCTATTGTTTTAACATTTGTT
GGGGGATTGGCGTTTTCTACTTCTGTTTTATCAAATATTGACAAAGCGAACGCCTATCGTTTGGTTTTTGTTATGGCTTT
TATAGCTTTATTTTTTGGGAATATTTTGTATCTGCTTTTTTCTTTTTTATCAAAAATATCCTTATCATCAAAAATATCCT
TATCAAAAGAGGAAAGAGATAAACAAGAAAATTTTTTCAAAAAACCTATTTTTTGGTTTAATCTAATGGTTACAATTCTA
TTTGTGATTGGTTTTTTTGGAGAATTGCATATAATACAAAGATTAGTTTCTAAATATCTTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT194803 WP_000581937.1 NZ_CP070962:648354-648602 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT194803 NZ_CP094082:17875-18312 [Helicobacter pylori]
ATGGTCAATACTTTGGAATTAGCAAAATATATTCTTAAGCATTCCAACAAAGAATTGAGCAACTTAGAATTGCAAAAAAC
TTTGTATTTCACAGAGCTTGACTATATTAAAAAGTTTGACAAACACCTAGTTTCTGATGATTTTGAAGCTTGGAAATACG
GGCCTGTTGCAAGAGAAGTGTATTATGAATATCGCAATTACGGTGCCAATTCTATTGACAAACCTAAAGAAGAAACGCTC
TCACAAAATCTTAGAGAAGATGAACTTGAAACCATTAATTGCTCCATAAAAAAATGCAACGAGAAATCCTATTGGGATTT
AGTGAAGGAGAGTCATAAAGAAGATGGCGCTTGGCATAAAAGCTTCAGAGAAGATAGAAAAGAAATCATTAGCAAAGATT
TGATCAAACAAGAAGCAAAACAAGCAAATGGAAACTAA

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKH
• PDB: 5CO7
• PDB: 3NFC
• PDB: 5CR2
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE
• PDB: 5CKF

Antitoxin

• AlphaFold DB: A0A7U9LMB4