Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 518623..519277 Replicon chromosome
Accession NZ_CP070962
Organism Escherichia coli strain K-12 J53

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JTR57_RS02595 Protein ID WP_000244777.1
Coordinates 518870..519277 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JTR57_RS02590 Protein ID WP_000354046.1
Coordinates 518623..518889 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTR57_RS02565 (513792) 513792..514535 + 744 WP_000951964.1 SDR family oxidoreductase -
JTR57_RS02570 (514592) 514592..516025 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
JTR57_RS02575 (516070) 516070..516381 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JTR57_RS02580 (516545) 516545..517204 + 660 WP_000250274.1 hemolysin III family protein -
JTR57_RS02585 (517400) 517400..518380 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JTR57_RS02590 (518623) 518623..518889 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JTR57_RS02595 (518870) 518870..519277 + 408 WP_000244777.1 protein YgfX Toxin
JTR57_RS02600 (519317) 519317..519838 - 522 WP_001055874.1 flavodoxin FldB -
JTR57_RS02605 (519950) 519950..520846 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JTR57_RS02610 (520871) 520871..521581 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JTR57_RS02615 (521587) 521587..523320 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T194802 WP_000244777.1 NZ_CP070962:518870-519277 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T194802 NZ_CP094080:618728-619012 [Helicobacter pylori]
ATGTATGCTTTAGCGTTTGATTTAAAGATTGAGATTTTAAAAAAAGAATACGGAGAACCCTACAATAAAGCCTATGATGA
TTTAAGGCAAGAATTAGAGCTATTAGGGTTTGAATGGACTCAAGGGAGCGTTTATGTTAATTATTCTAAAGAAAACACTC
TAGCACAAGTCTATAAAGCGATCAATAAACTCTCTCAAATTGAGTGGTTTAAAAAGTCTGTTAGGGATATTAGAGCGTTT
AAGGTAGAGGACTTTAGCGATTTTACTGAGATTGTGAAATCCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT194802 WP_000354046.1 NZ_CP070962:518623-518889 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT194802 NZ_CP094080:618355-618747 [Helicobacter pylori]
ATGCCTAATTCTCCCACCAAAAAAGACTACACGCAATACAGCGAAAAACAGCTTTTTAACTTAATCAACCAATTAGAGCA
AAAGATCAAAAAGATGCAAAATGATAGAGCTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCATTAAATGAACTCTTGCAAAAAATCAGCCAAGCTTTTGACGATAAAAGAGATTGTTGTTTG
GGGCGCAAGATCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGTTAAATGGGGTTAATCTCACTCAAATTGATAG
TTTAGATGATCTTACAAACGAGTTAAAAAGAGAAAATGGTAAAGGTTTTGAAAATGTATGCTTTAGCGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References