Detailed information of TA system TA194786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 1137395..1138051
• Replicon: chromosome
• Accession: NZ_CP070959
• Organism: Thiomicrospira microaerophila strain BDL05

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: JX580_RS05435
• Protein ID: WP_248851782.1
• Coordinates: 1137395..1137574 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JX580_RS05440
• Protein ID: WP_248851783.1
• Coordinates: 1137641..1138051 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JX580_RS05405 (JX580_05415)	1132944..1133777	+	834	WP_248851776.1	ABC transporter permease	-
JX580_RS05410 (JX580_05420)	1133845..1134054	-	210	WP_248851777.1	Rho-binding antiterminator	-
JX580_RS05415 (JX580_05425)	1134103..1135791	+	1689	WP_248851778.1	sigma 54-interacting transcriptional regulator	-
JX580_RS05420 (JX580_05430)	1135910..1136143	+	234	WP_248851779.1	type II toxin-antitoxin system VapB family antitoxin	-
JX580_RS05425 (JX580_05435)	1136136..1136558	+	423	WP_248851780.1	type II toxin-antitoxin system VapC family toxin	-
JX580_RS05430 (JX580_05440)	1136851..1137207	+	357	WP_248851781.1	hypothetical protein	-
JX580_RS05435 (JX580_05445)	1137395..1137574	+	180	WP_248851782.1	type II toxin-antitoxin system HicA family toxin	Toxin
JX580_RS05440 (JX580_05450)	1137641..1138051	+	411	WP_248851783.1	hypothetical protein	Antitoxin
JX580_RS05445 (JX580_05455)	1138076..1139287	-	1212	WP_248851887.1	FAD-dependent oxidoreductase	-
JX580_RS05450	1139276..1139446	-	171	Protein_1052	rubredoxin	-
JX580_RS05455 (JX580_05460)	1139455..1140531	-	1077	WP_248851784.1	acyl-CoA dehydrogenase family protein	-
JX580_RS05460 (JX580_05465)	1140580..1141116	-	537	WP_248851785.1	OsmC family protein	-
JX580_RS05465 (JX580_05470)	1141448..1141891	+	444	WP_248851786.1	cyanase	-
JX580_RS05470 (JX580_05475)	1142076..1143035	+	960	WP_248851787.1	SLAC1 anion channel family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-58)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-132)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6711.95 Da
• Isoelectric Point: 10.6579

>T194786 WP_248851782.1 NZ_CP070959:1137395-1137574 [Thiomicrospira microaerophila]
MKSSELRKWLIEQGARFEKAKGSHFKVYLGDKMTIFPFHGAKEMATGTVNKILKDLGLK

Nucleotide

• Length: 180 bp

>T194786 NZ_CP094065:1625431-1625712 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCTGGGCGTATTATAGAAAGCAA
TATTGCAAGCGTTGTTGATTGCCTCAAAGAGCAAAAACCACTCCAACAAAAATATTGCGACCATGCCTTGAGTGGCAATC
TTAAAGGGTTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14857.16 Da
• Isoelectric Point: 6.0832

>AT194786 WP_248851783.1 NZ_CP070959:1137641-1138051 [Thiomicrospira microaerophila]
MQYAMNLEKEGDILIVTFPDIPEALTEVHKGADLQAMALDALESALDFYPETKRPIPLPSKTNAANGLLIPTALAAKIHL
YNAWLASGQSKTELATKVGVAPSNLNRLFDFKYRSKIEAIEAALHALGKHFELQVA

Nucleotide

• Length: 411 bp

>AT194786 NZ_CP094065:1625124-1625438 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAACAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTGAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA