Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 33731..34256 Replicon plasmid pTB44P4
Accession NZ_CP070958
Organism Escherichia fergusonii strain EF44

Toxin (Protein)


Gene name ccdB Uniprot ID F0JY91
Locus tag JYG65_RS23200 Protein ID WP_001159869.1
Coordinates 33951..34256 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag JYG65_RS23195 Protein ID WP_000813630.1
Coordinates 33731..33949 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JYG65_RS23290 29419..29569 - 151 Protein_30 hypothetical protein -
JYG65_RS23175 (JYG65_23075) 29522..29752 - 231 WP_252990174.1 IS66 family insertion sequence element accessory protein TnpB -
JYG65_RS23180 30630..31280 + 651 Protein_32 transposase -
JYG65_RS23185 (JYG65_23085) 31558..32154 - 597 Protein_33 IS66 family transposase -
JYG65_RS23190 (JYG65_23090) 32244..32672 - 429 Protein_34 hypothetical protein -
JYG65_RS23195 (JYG65_23095) 33731..33949 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JYG65_RS23200 (JYG65_23100) 33951..34256 + 306 WP_001159869.1 type II toxin-antitoxin system toxin CcdB Toxin
JYG65_RS23205 (JYG65_23105) 34257..34457 + 201 Protein_37 phage integrase family protein -
JYG65_RS23210 (JYG65_23110) 34442..35353 - 912 WP_000549737.1 DMT family transporter -
JYG65_RS23215 (JYG65_23115) 35470..35607 + 138 Protein_39 Lrp/AsnC family transcriptional regulator -
JYG65_RS23220 (JYG65_23120) 35607..36656 + 1050 Protein_40 IS4 family transposase -
JYG65_RS23225 (JYG65_23125) 36756..38129 - 1374 WP_000244368.1 IS4-like element ISEc13 family transposase -
JYG65_RS23230 (JYG65_23130) 38341..38775 - 435 WP_000525034.1 IS200/IS605 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..46743 46743
- inside IScluster/Tn - - 28571..38775 10204


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11678.46 Da        Isoelectric Point: 6.4674

>T194783 WP_001159869.1 NZ_CP070958:33951-34256 [Escherichia fergusonii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTSDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T194783 NZ_CP094064:578927-579193 [Helicobacter pylori]
GTGTTGAAACTCAATCTTAAAAAATCTTTTCAAAAAGATTTTGATAAATTACTTTTGAATGGGTTTGATGATAGCGTTTT
GAATAAGGTCATTCTAACCTTAAGAAAAAAAGAACCGCTAGATCCACAATTTCAAGATCATGCTTTAAAAGGAAAGTGGA
AACCTTTTAGGGAATGCCACATTAAGCCTGATATTTTGCTTGTGTATTTAGTGAAAGATGATGAGCTTATTTTAGTAAGA
CTAGGCAGTCATAGCGAGCTGTTTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT194783 WP_000813630.1 NZ_CP070958:33731-33949 [Escherichia fergusonii]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT194783 NZ_CP094064:578569-578946 [Helicobacter pylori]
ATGCCTAACTCTCCCACTAAAAAAGACTACACACAATACAGCGAAAAACAGCTTTTTAACTTAATCAACCAATTAGAGCA
AAAGATCAAAAAGATGCAAAATGATAGAGCTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCATTGAATGGACTCTTGCAAAAAATCAGCCAAGCTTTTGATGATAAAAGAGATTGTTGTTTG
GGGTGCAAGATCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGCTCAACAAAGAAACCGATTTGATTGTTGATGA
TTTTTCTAGTTATAGCGATGAAAGAAAAAAGGCGTTAGGTGTTGAAACTCAATCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3V2SIB9

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References