Detailed information of TA system TA194779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 77787..78334
• Replicon: plasmid pTB44P1
• Accession: NZ_CP070955
• Organism: Escherichia fergusonii strain EF44

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: JYG65_RS21715
• Protein ID: WP_244442846.1
• Coordinates: 77787..78065 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: JYG65_RS21720
• Protein ID: WP_000079941.1
• Coordinates: 78065..78334 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYG65_RS21685 (JYG65_21605)	73020..74060	-	1041	Protein_84	protein YagA	-
JYG65_RS21690 (JYG65_21610)	74414..75016	+	603	WP_000517695.1	ProQ/FINO family protein	-
JYG65_RS21695 (JYG65_21615)	75072..75566	+	495	WP_001459505.1	hypothetical protein	-
JYG65_RS21700 (JYG65_21620)	75566..75832	+	267	WP_000955263.1	hypothetical protein	-
JYG65_RS21705 (JYG65_21625)	76309..76782	-	474	WP_001334658.1	hypothetical protein	-
JYG65_RS21710 (JYG65_21630)	76899..77747	-	849	WP_001057996.1	3'-5' exonuclease	-
JYG65_RS21715 (JYG65_21635)	77787..78065	-	279	WP_244442846.1	type II toxin-antitoxin system toxin YacB	Toxin
JYG65_RS21720 (JYG65_21640)	78065..78334	-	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
JYG65_RS21725 (JYG65_21645)	78389..78547	-	159	WP_000841008.1	hypothetical protein	-
JYG65_RS21730 (JYG65_21650)	79243..80274	-	1032	WP_000907873.1	plasmid replication initiator RepA	-
JYG65_RS21735 (JYG65_21655)	81232..81510	+	279	WP_219065241.1	ash family protein	-
JYG65_RS21740 (JYG65_21660)	81428..81715	+	288	WP_000074849.1	conjugal transfer protein TraA	-
JYG65_RS21745 (JYG65_21665)	82157..82690	+	534	WP_001303319.1	transcription termination/antitermination NusG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / sitABCD / aadA5	-	1..143778	143778

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10761.43 Da
• Isoelectric Point: 9.7617

>T194779 WP_244442846.1 NZ_CP070955:c78065-77787 [Escherichia fergusonii]
MEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T194779 NZ_CP094061:754914-755195 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTACAGAAAGCGA
TGTTACGAGCGTTGTTGATTGTCTCAAAGAGCAAAAACCACTCCAACAAAAATATTGCGACCATGCCTTAAGTGGCAATC
TTAAAGGGTTGAGAGAATGCCATGTTAAGCCAAACTTGTTATTGATTTATGAAGTCAAAAAACAAGAAAATGAACTTGTT
TTATTGCGCCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT194779 WP_000079941.1 NZ_CP070955:c78334-78065 [Escherichia fergusonii]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT194779 NZ_CP094061:754607-754921 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACGCTATTGAAAAACAATG
GCTTAAGGCAGAAAGGGATAAAAACAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAACACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5