Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 82244..83867 Replicon plasmid pTB31P1
Accession NZ_CP070952
Organism Escherichia fergusonii strain EF31

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag JW961_RS21890 Protein ID WP_000088046.1
Coordinates 82542..83867 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag JW961_RS21885 Protein ID WP_024136327.1
Coordinates 82244..82537 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW961_RS21860 (JW961_21765) 78116..79357 + 1242 WP_001053338.1 VWA domain-containing protein -
JW961_RS23140 79448..79579 + 132 WP_000374059.1 hypothetical protein -
JW961_RS21865 (JW961_21770) 79920..80201 - 282 WP_000077926.1 hypothetical protein -
JW961_RS21870 (JW961_21775) 80251..80442 - 192 WP_000398480.1 hypothetical protein -
JW961_RS21875 (JW961_21780) 80534..80875 - 342 WP_001151575.1 hypothetical protein -
JW961_RS21880 (JW961_21785) 81248..81640 + 393 WP_000341066.1 hypothetical protein -
JW961_RS21885 (JW961_21790) 82244..82537 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
JW961_RS21890 (JW961_21795) 82542..83867 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
JW961_RS21895 (JW961_21800) 83928..84134 + 207 WP_062914745.1 hypothetical protein -
JW961_RS21900 (JW961_21805) 84236..84646 + 411 WP_000985911.1 hypothetical protein -
JW961_RS21905 (JW961_21810) 84659..85474 + 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
JW961_RS21910 (JW961_21815) 85728..86153 + 426 WP_001043843.1 hypothetical protein -
JW961_RS21915 (JW961_21820) 86702..87010 + 309 WP_001224687.1 hypothetical protein -
JW961_RS21920 (JW961_21825) 87026..87883 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / tet(M) / tet(A) / dfrA14 / sul3 / blaTEM-1B / oqxB / oqxA / qnrS1 - 1..184652 184652


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T194749 WP_000088046.1 NZ_CP070952:82542-83867 [Escherichia fergusonii]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T194749 NZ_CP093957:c1330092-1329748 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTAGAAGAATATAATGTGGAGTTAGTTTTTACTACTTTGAACAAACGCGCATGTTTCGA
CCCTACCTACGGTATCATATTTGTAAATCAAAATTTAACACCATCAGAACAAGAAGAAGCAATATATCACGAATTAAAGC
ATGTAAAAGACCATGTGGATATAATGGCATTGTATAAAATTCCTGTTTTTCGTTCTAAGATGGAAGCTGAAGCAGAACAA
TATATGTTTAGAAGCTTAATCGAAAAATATGAAGGACAATACAATTACTCAAATATTATAGCTCATTACAACTTAAAAAT
GGGACAAGAAATTTATTTGAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT194749 WP_024136327.1 NZ_CP070952:82244-82537 [Escherichia fergusonii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT194749 NZ_CP093957:c1330434-1330111 [Enterococcus faecalis]
ATGACTGTATTTGATAGAGTAAAAAAATTAGCAGATAGTCAGAAAATATCTATTGTCGAACTTGAAGAAAAGTTAAATTT
CAGTCGAAATTCATTATACGCTTGGAAAAAAAGTAAACCTTCTATTGATAAATTAGAAGCTGTTGCAAATTATTTTGGAG
TTTCAACGGATTATTTATTAGGTCGTGAAGTTTCTAATAAATCAAAGCAATCTGATGATTTGGATGAAGTATTAGACAAT
GTGATGAGTTTCGATGGTGAACCTTTGGATGATCATGATCGGGAAGTTATTCGTGCATATTTAAAGGGTAGATTCGGGAA
ATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References