Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1346405..1347030 Replicon chromosome
Accession NZ_CP070951
Organism Escherichia fergusonii strain EF31

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag JW961_RS06375 Protein ID WP_000911329.1
Coordinates 1346632..1347030 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag JW961_RS06370 Protein ID WP_000450524.1
Coordinates 1346405..1346632 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW961_RS06345 (1342207) 1342207..1342677 - 471 WP_001068688.1 thioredoxin-dependent thiol peroxidase -
JW961_RS06350 (1342677) 1342677..1343249 - 573 WP_000189057.1 glycine cleavage system transcriptional repressor -
JW961_RS06355 (1343396) 1343396..1344274 + 879 WP_000494011.1 4-hydroxy-tetrahydrodipicolinate synthase -
JW961_RS06360 (1344291) 1344291..1345325 + 1035 WP_002431657.1 outer membrane protein assembly factor BamC -
JW961_RS06365 (1345537) 1345537..1346250 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
JW961_RS06370 (1346405) 1346405..1346632 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
JW961_RS06375 (1346632) 1346632..1347030 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
JW961_RS06380 (1347177) 1347177..1348040 + 864 WP_160523810.1 neutral zinc metallopeptidase -
JW961_RS06385 (1348055) 1348055..1350070 + 2016 WP_223663423.1 tRNA cytosine(34) acetyltransferase TmcA -
JW961_RS06390 (1350144) 1350144..1350842 + 699 WP_000679802.1 esterase -
JW961_RS06395 (1350935) 1350935..1351135 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T194731 WP_000911329.1 NZ_CP070951:1346632-1347030 [Escherichia fergusonii]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T194731 NZ_CP093947:195967-196233 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT194731 WP_000450524.1 NZ_CP070951:1346405-1346632 [Escherichia fergusonii]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT194731 NZ_CP093947:195704-195967 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References