Detailed information of TA system TA194730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 927305..927888
• Replicon: chromosome
• Accession: NZ_CP070951
• Organism: Escherichia fergusonii strain EF31

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: V0SV58
• Locus tag: JW961_RS04425
• Protein ID: WP_000254750.1
• Coordinates: 927553..927888 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JW961_RS04420
• Protein ID: WP_223662808.1
• Coordinates: 927305..927553 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW961_RS04410 (923644)	923644..924942	+	1299	WP_000046872.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
JW961_RS04415 (924993)	924993..927227	+	2235	WP_000226844.1	GTP diphosphokinase	-
JW961_RS04420 (927305)	927305..927553	+	249	WP_223662808.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JW961_RS04425 (927553)	927553..927888	+	336	WP_000254750.1	endoribonuclease MazF	Toxin
JW961_RS04430 (927959)	927959..928750	+	792	WP_223662810.1	nucleoside triphosphate pyrophosphohydrolase	-
JW961_RS04435 (928978)	928978..930615	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
JW961_RS04440 (930703)	930703..932001	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• Antitoxin PemI-like (2-82)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12067.95 Da
• Isoelectric Point: 8.2618

>T194730 WP_000254750.1 NZ_CP070951:927553-927888 [Escherichia fergusonii]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T194730 NZ_CP093942:c170063-169911 [Enterococcus faecium]
ATTGAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATTTCATCATCATCTTTATAAGGATGGTGTTAGAATCACTGT
TCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGCGAAAAATTTTAAAGGATGCGGGATTGAAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9297.70 Da
• Isoelectric Point: 4.5972

>AT194730 WP_223662808.1 NZ_CP070951:927305-927553 [Escherichia fergusonii]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVGGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT194730 NZ_CP093942:c169877-169446 [Enterococcus faecium]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGAATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGATCTACTTCCAGAACCAACACCAATCAATAAGATTTCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGCTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG

Structures

Toxin

• AlphaFold DB: V0SV58