Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 684650..685200 Replicon chromosome
Accession NZ_CP070951
Organism Escherichia fergusonii strain EF31

Toxin (Protein)


Gene name ccdB Uniprot ID F0JPU1
Locus tag JW961_RS03320 Protein ID WP_001160963.1
Coordinates 684886..685200 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag JW961_RS03315 Protein ID WP_000125566.1
Coordinates 684650..684883 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW961_RS03290 (679722) 679722..680009 + 288 WP_000203741.1 ferredoxin-like protein FixX -
JW961_RS03295 (680068) 680068..681399 + 1332 WP_001183184.1 MFS transporter -
JW961_RS03300 (681507) 681507..682037 + 531 WP_001619100.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
JW961_RS03305 (682030) 682030..683892 + 1863 WP_000377102.1 glutathione-regulated potassium-efflux system protein KefC -
JW961_RS03310 (684085) 684085..684564 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
JW961_RS03315 (684650) 684650..684883 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
JW961_RS03320 (684886) 684886..685200 + 315 WP_001160963.1 CcdB family protein Toxin
JW961_RS03325 (685197) 685197..686045 - 849 WP_000257187.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
JW961_RS03330 (686052) 686052..686429 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
JW961_RS03335 (686432) 686432..687253 - 822 WP_001065388.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
JW961_RS03340 (687250) 687250..688239 - 990 WP_104918215.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
JW961_RS03345 (688239) 688239..689525 - 1287 WP_223663088.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11722.70 Da        Isoelectric Point: 8.0666

>T194729 WP_001160963.1 NZ_CP070951:684886-685200 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T194729 NZ_CP093941:535069-535410 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT194729 WP_000125566.1 NZ_CP070951:684650-684883 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT194729 NZ_CP093941:534839-535069 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F0JPU1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References