Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4200881..4201378 Replicon chromosome
Accession NZ_CP070930
Organism Escherichia coli strain 30COLEC

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JR883_RS20150 Protein ID WP_001173343.1
Coordinates 4200881..4201054 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JR883_RS20155 Protein ID WP_000398619.1
Coordinates 4201082..4201378 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR883_RS20140 4197551..4199008 + 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -
JR883_RS20145 4199245..4200762 + 1518 WP_001295087.1 lysine--tRNA ligase -
JR883_RS20150 4200881..4201054 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JR883_RS20155 4201082..4201378 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JR883_RS20160 4201605..4201877 - 273 WP_000405647.1 N-acetyltransferase -
JR883_RS20165 4201889..4202119 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
JR883_RS20170 4202300..4203931 + 1632 WP_001216477.1 sensor histidine kinase -
JR883_RS20175 4203928..4204647 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4184125..4201378 17253


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T194724 WP_001173343.1 NZ_CP070930:c4201054-4200881 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T194724 NZ_CP093935:c2526693-2526586 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT194724 WP_000398619.1 NZ_CP070930:c4201378-4201082 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT194724 NZ_CP093935:2526509-2526569 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References