Detailed information of TA system TA194714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2286397..2286923
• Replicon: chromosome
• Accession: NZ_CP070930
• Organism: Escherichia coli strain 30COLEC

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: JR883_RS10945
• Protein ID: WP_000323025.1
• Coordinates: 2286636..2286923 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: JR883_RS10940
• Protein ID: WP_000534858.1
• Coordinates: 2286397..2286636 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR883_RS10910	2281495..2281737	+	243	Protein_2132	DUF977 family protein	-
JR883_RS10915	2281734..2282090	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
JR883_RS10920	2282701..2282988	+	288	Protein_2134	hypothetical protein	-
JR883_RS10925	2283757..2285070	+	1314	WP_000589005.1	ISNCY family transposase	-
JR883_RS10930	2285532..2285864	-	333	WP_001301033.1	protein FlxA	-
JR883_RS10935	2286067..2286372	-	306	WP_001326990.1	hypothetical protein	-
JR883_RS10940	2286397..2286636	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
JR883_RS10945	2286636..2286923	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
JR883_RS10950	2286995..2287150	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
JR883_RS10955	2287367..2287618	+	252	WP_000980994.1	hypothetical protein	-
JR883_RS10960	2287685..2287963	+	279	WP_012304870.1	hypothetical protein	-
JR883_RS10965	2287965..2289014	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
JR883_RS10970	2289028..2289780	+	753	WP_196611805.1	antitermination protein	-
JR883_RS10975	2290058..2290147	-	90	WP_120795389.1	hypothetical protein	-
JR883_RS10980	2290202..2290414	-	213	WP_000087756.1	cold shock-like protein CspF	-
JR883_RS10985	2290715..2290930	+	216	WP_000066484.1	cold shock-like protein CspB	-
JR883_RS10990	2291684..2291899	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	mcr-1.1	-	2254133..2304009	49876

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T194714 WP_000323025.1 NZ_CP070930:2286636-2286923 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T194714 NZ_CP093935:c836818-836630 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT194714 WP_000534858.1 NZ_CP070930:2286397-2286636 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT194714 NZ_CP093935:c837107-836847 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8