194697

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 67291..67892 Replicon plasmid p40EC-3
Accession NZ_CP070923
Organism Escherichia coli strain 40EC

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag JR909_RS24690 Protein ID WP_001216034.1
Coordinates 67291..67671 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag JR909_RS24695 Protein ID WP_001190712.1
Coordinates 67671..67892 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR909_RS24655 63414..64111 - 698 Protein_62 IS1 family transposase -
JR909_RS24660 64282..64491 + 210 WP_000874156.1 hypothetical protein -
JR909_RS24665 64602..65453 + 852 WP_000611664.1 hypothetical protein -
JR909_RS24670 65486..65848 - 363 Protein_65 phage antirepressor KilAC domain-containing protein -
JR909_RS24675 65904..66601 + 698 Protein_66 IS1 family transposase -
JR909_RS24680 66615..67079 - 465 Protein_67 hypothetical protein -
JR909_RS24685 67107..67286 - 180 WP_038997082.1 hypothetical protein -
JR909_RS24690 67291..67671 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JR909_RS24695 67671..67892 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JR909_RS24700 68075..69631 + 1557 WP_038997084.1 type I restriction-modification system subunit M -
JR909_RS24705 69628..70866 + 1239 WP_038997080.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..102703 102703
- flank IS/Tn - - 66098..66601 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T194697 WP_001216034.1 NZ_CP070923:c67671-67291 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T194697 NZ_CP093930:c2180575-2180366 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT194697 WP_001190712.1 NZ_CP070923:c67892-67671 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT194697 NZ_CP093930:c2180880-2180587 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References