Detailed information of TA system TA194686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 62895..63442
• Replicon: plasmid p40EC-1
• Accession: NZ_CP070921
• Organism: Escherichia coli strain 40EC

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: JR909_RS23350
• Protein ID: WP_000969990.1
• Coordinates: 62895..63176 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A075M9N8
• Locus tag: JR909_RS23355
• Protein ID: WP_032072813.1
• Coordinates: 63173..63442 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR909_RS23330	60574..60735	+	162	WP_000251882.1	hypothetical protein	-
JR909_RS23335	60753..61100	-	348	WP_000142424.1	hypothetical protein	-
JR909_RS23340	61402..61884	-	483	WP_001311056.1	hypothetical protein	-
JR909_RS23345	62001..62849	-	849	WP_023154360.1	3'-5' exonuclease	-
JR909_RS23350	62895..63176	-	282	WP_000969990.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JR909_RS23355	63173..63442	-	270	WP_032072813.1	prevent host death protein Phd antitoxin	Antitoxin
JR909_RS23360	64357..65214	-	858	WP_000130940.1	incFII family plasmid replication initiator RepA	-
JR909_RS23365	65207..65281	-	75	WP_001365705.1	RepA leader peptide Tap	-
JR909_RS23370	65507..65767	-	261	WP_000083818.1	replication regulatory protein RepA	-
JR909_RS23375	66006..66579	-	574	Protein_58	DUF2726 domain-containing protein	-
JR909_RS23380	66617..66826	-	210	WP_001333231.1	hemolysin expression modulator Hha	-
JR909_RS23385	66872..67333	-	462	Protein_60	thermonuclease family protein	-
JR909_RS23390	67576..67788	-	213	WP_012311405.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN	1..131966	131966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10930.61 Da
• Isoelectric Point: 8.4615

>T194686 WP_000969990.1 NZ_CP070921:c63176-62895 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTVQKWP

Nucleotide

• Length: 282 bp

>T194686 NZ_CP093926:c1342426-1342274 [Enterococcus hirae]
ATTGAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATCTCATCATCATCTTTATAAGGATGGTGTTAGAATCACTGT
TCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGCAAAAAATTTTAAAGGATGCGGGATTGAAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10277.64 Da
• Isoelectric Point: 10.2879

>AT194686 WP_032072813.1 NZ_CP070921:c63442-63173 [Escherichia coli]
MAQVNMNLRINAEIKNAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT194686 NZ_CP093926:c1342240-1341809 [Enterococcus hirae]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGAATTGCTATGGCACAAGAGGTTTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGAGCTACTTCCAGAACCAACACCAATCAATAAGATCTCTGTTAAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGTTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG

Structures

Antitoxin

• AlphaFold DB: A0A075M9N8