194683

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 4145625..4146960 Replicon chromosome
Accession NZ_CP070920
Organism Escherichia coli strain 40EC

Toxin (Protein)


Gene name sanaT Uniprot ID F4VMR9
Locus tag JR909_RS20130 Protein ID WP_000342497.1
Coordinates 4145625..4146572 (-) Length 316 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID E0J2C6
Locus tag JR909_RS20135 Protein ID WP_000152741.1
Coordinates 4146565..4146960 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR909_RS20105 4141478..4142194 + 717 WP_001295734.1 N-acetylneuraminic acid outer membrane channel NanC -
JR909_RS20110 4142214..4143320 + 1107 WP_001542605.1 N-acetylneuraminate epimerase -
JR909_RS20115 4143385..4144365 + 981 WP_001542604.1 transposase -
JR909_RS20120 4144373..4145023 - 651 WP_001037978.1 HNH endonuclease -
JR909_RS20125 4145402..4145587 + 186 WP_000066585.1 hypothetical protein -
JR909_RS20130 4145625..4146572 - 948 WP_000342497.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
JR909_RS20135 4146565..4146960 - 396 WP_000152741.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
JR909_RS20140 4147030..4147863 - 834 WP_001192743.1 DUF4942 domain-containing protein -
JR909_RS20145 4148169..4149725 + 1557 WP_000202821.1 type I restriction-modification system subunit M -
JR909_RS20150 4149722..4150996 + 1275 WP_001293046.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimC / fimI / fimA / fimE / fimB 4134616..4150996 16380


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-303)

Antitoxin

Protein of unknown function DUF6088

(7-108)


Sequences

Toxin        


Download         Length: 316 a.a.        Molecular weight: 35588.79 Da        Isoelectric Point: 4.9737

>T194683 WP_000342497.1 NZ_CP070920:c4146572-4145625 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Download         Length: 948 bp

>T194683 NZ_CP093920:86335-86484 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14619.93 Da        Isoelectric Point: 11.6915

>AT194683 WP_000152741.1 NZ_CP070920:c4146960-4146565 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRELVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Download         Length: 396 bp

>AT194683 NZ_CP093920:c86291-86230 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L5BAQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A1L4ZXD4

References