Detailed information of TA system TA194680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2427449..2428087
• Replicon: chromosome
• Accession: NZ_CP070920
• Organism: Escherichia coli strain 40EC

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JR909_RS11740
• Protein ID: WP_000813794.1
• Coordinates: 2427911..2428087 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JR909_RS11735
• Protein ID: WP_001270286.1
• Coordinates: 2427449..2427865 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR909_RS11715	2422601..2423542	-	942	WP_001251313.1	ABC transporter permease	-
JR909_RS11720	2423543..2424556	-	1014	WP_001542893.1	ABC transporter ATP-binding protein	-
JR909_RS11725	2424574..2425719	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
JR909_RS11730	2425964..2427370	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
JR909_RS11735	2427449..2427865	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JR909_RS11740	2427911..2428087	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JR909_RS11745	2428309..2428539	+	231	WP_000494244.1	YncJ family protein	-
JR909_RS11750	2428631..2430592	-	1962	WP_001542890.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JR909_RS11755	2430665..2431201	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
JR909_RS11760	2431254..2432474	+	1221	WP_205899196.1	benzoate/H(+) symporter BenE family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2432514..2433662	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T194680 WP_000813794.1 NZ_CP070920:c2428087-2427911 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T194680 NZ_CP070920:c2428087-2427911 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT194680 WP_001270286.1 NZ_CP070920:c2427865-2427449 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT194680 NZ_CP070920:c2427865-2427449 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB