194660

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3495798..3496635 Replicon chromosome
Accession NZ_CP070914
Organism Escherichia coli strain 65COLEC

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag JR892_RS16915 Protein ID WP_000227784.1
Coordinates 3496093..3496635 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag JR892_RS16910 Protein ID WP_001297137.1
Coordinates 3495798..3496109 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR892_RS16885 3490818..3491765 + 948 WP_001239434.1 cytochrome o ubiquinol oxidase subunit II -
JR892_RS16890 3491787..3493778 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JR892_RS16895 3493768..3494382 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JR892_RS16900 3494382..3494711 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JR892_RS16905 3494723..3495613 + 891 WP_000971336.1 heme o synthase -
JR892_RS16910 3495798..3496109 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
JR892_RS16915 3496093..3496635 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
JR892_RS16920 3496691..3497626 - 936 WP_053900775.1 sel1 repeat family protein -
JR892_RS16925 3498034..3499398 + 1365 WP_001000978.1 MFS transporter -
JR892_RS16930 3499526..3500017 - 492 WP_001138904.1 nucleotide binding protein YajQ -
JR892_RS16935 3500185..3501096 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T194660 WP_000227784.1 NZ_CP070914:3496093-3496635 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T194660 NZ_CP093919:185985-186092 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT194660 WP_001297137.1 NZ_CP070914:3495798-3496109 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT194660 NZ_CP093919:c185937-185871 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References