Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3461720..3462338 Replicon chromosome
Accession NZ_CP070914
Organism Escherichia coli strain 65COLEC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JR892_RS16745 Protein ID WP_001291435.1
Coordinates 3462120..3462338 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JR892_RS16740 Protein ID WP_000344800.1
Coordinates 3461720..3462094 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR892_RS16730 3456809..3458002 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JR892_RS16735 3458025..3461174 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
JR892_RS16740 3461720..3462094 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JR892_RS16745 3462120..3462338 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JR892_RS16750 3462510..3463061 + 552 WP_000102564.1 maltose O-acetyltransferase -
JR892_RS16755 3463177..3463647 + 471 WP_000136192.1 YlaC family protein -
JR892_RS16760 3463811..3465361 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JR892_RS16765 3465403..3465756 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JR892_RS16775 3466135..3466446 + 312 WP_000409911.1 MGMT family protein -
JR892_RS16780 3466477..3467049 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T194659 WP_001291435.1 NZ_CP070914:3462120-3462338 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T194659 NZ_CP093916:27911-28216 [Enterobacter cloacae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT194659 WP_000344800.1 NZ_CP070914:3461720-3462094 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT194659 NZ_CP093916:27691-27909 [Enterobacter cloacae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References