Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 869317..869971 Replicon chromosome
Accession NZ_CP070914
Organism Escherichia coli strain 65COLEC

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JR892_RS04195 Protein ID WP_000244777.1
Coordinates 869564..869971 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JR892_RS04190 Protein ID WP_000354046.1
Coordinates 869317..869583 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR892_RS04165 864487..865230 + 744 WP_000951962.1 SDR family oxidoreductase -
JR892_RS04170 865287..866720 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
JR892_RS04175 866765..867076 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
JR892_RS04180 867239..867898 + 660 WP_000250274.1 hemolysin III family protein -
JR892_RS04185 868094..869074 - 981 WP_001625883.1 tRNA-modifying protein YgfZ -
JR892_RS04190 869317..869583 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JR892_RS04195 869564..869971 + 408 WP_000244777.1 toxin CptA Toxin
JR892_RS04200 870011..870532 - 522 WP_001055874.1 flavodoxin FldB -
JR892_RS04205 870644..871540 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JR892_RS04210 871565..872275 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JR892_RS04215 872281..874014 + 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T194646 WP_000244777.1 NZ_CP070914:869564-869971 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T194646 NZ_CP093913:c1109359-1109063 [Aggregatibacter actinomycetemcomitans]
ATGGGTGAACAATTGAAAGATTTTGATCCTGCAGAATATTTATCTACCGAAGAAGATATTCAGCTTTATTTAAATGAAGT
GCTACAAGAAAATAATATTGAGCTTATTTTGTCTGCGTTAGGCGATATCGCACGAACCAGAAATATGAGCCAAATTGCCC
GCGAAACCGGTATGAGTAGAGAAGGGCTTTATAAAGCCCTGTCCGGTACGGGCAATCCTACCTTTGCCACCGTGATTAAA
GTCATGAAAGCCTTAAATTTACAACTCCAAGTGCAACCTTCCGGTTACGCTTCTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT194646 WP_000354046.1 NZ_CP070914:869317-869583 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT194646 NZ_CP093913:c1109655-1109344 [Aggregatibacter actinomycetemcomitans]
ATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAAT
CAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGA
TTGATGAAGGTCAGGGGTATCGAGTTTATCTGAAAAATCAAAATGGCATACTTGTTATCTTGCTTTGTGGCGGAGACAAA
TCCACTCAAGAGAAAGATATCAAGAAAGCAAAACAACTTGCACAGGAAATGGGACTATGGGTGAACAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References