Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 34131..34929 Replicon plasmid p88COLEC-2
Accession NZ_CP070908
Organism Escherichia coli strain 88COLEC

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag JR901_RS24105 Protein ID WP_000072677.1
Coordinates 34408..34929 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag JR901_RS24100 Protein ID WP_001351987.1
Coordinates 34131..34400 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR901_RS24075 29667..31007 - 1341 WP_000137333.1 AAA family ATPase -
JR901_RS24080 31051..31791 - 741 WP_063277813.1 hypothetical protein -
JR901_RS24085 31985..32740 - 756 WP_021533207.1 DUF2971 domain-containing protein -
JR901_RS24090 32785..33138 - 354 WP_160378290.1 hypothetical protein -
JR901_RS24095 33144..33812 - 669 WP_000161229.1 AAA family ATPase -
JR901_RS24100 34131..34400 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
JR901_RS24105 34408..34929 + 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
JR901_RS24110 35098..35349 - 252 WP_000901559.1 hypothetical protein -
JR901_RS24115 35351..36043 - 693 WP_205899153.1 hypothetical protein -
JR901_RS24120 36057..36380 - 324 WP_072651717.1 hypothetical protein -
JR901_RS24125 36583..39153 - 2571 WP_205899154.1 tail fiber protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..111012 111012


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T194641 WP_000072677.1 NZ_CP070908:34408-34929 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T194641 NZ_CP093913:c705223-704939 [Aggregatibacter actinomycetemcomitans]
ATGAGTTACATCATTGAGTTTATCCCATCATCTGAAAAAGAATTCCGCAAACTCGATATTAATCTGCGTAAACAATTCAT
TGAGAAATTAAAAGAACGCGCCGAAAATCCCCGAGTGTCATCCGCCAAATTACACGGCATGAAAGATTGCTATAAAATCA
AATTACGCAATGTAGGGTATCATTTGGTGTATCAGGTTATTGATGAACGCATTGTGGTCAAAGCCATCTCCGTAGGAAAA
CGCGATCGAAATTTTGTTTATACCAAAGCAAAAAGCAGGCTTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT194641 WP_001351987.1 NZ_CP070908:34131-34400 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp

>AT194641 NZ_CP093913:c705474-705220 [Aggregatibacter actinomycetemcomitans]
ATGACAAGCCTTGTTTTAACCGATGTTGTCGCCAGTATCACCGAATTAAAAACTAATCCCATGGCGGTACTTCGTGAAGC
CGGTGGAAAACCGCTGGCAATTTTAAACCGCAATGAACCCGCATTTTATTGCGTCCCACCGGCAATTTATGAATATTTGA
TGGAATTAGTCGATGATGCTGAATTGACAAAAATCGTGGAAGAACGTCAACATGAAAAAAGTTATCCTGTTGATTTAGAG
GATTTACTACAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References