Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 68139..68661 Replicon plasmid p88COLEC-1
Accession NZ_CP070907
Organism Escherichia coli strain 88COLEC

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag JR901_RS23515 Protein ID WP_000220560.1
Coordinates 68139..68420 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag JR901_RS23520 Protein ID WP_000121743.1
Coordinates 68410..68661 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR901_RS23505 65360..66748 - 1389 WP_001207923.1 nitrate/nitrite transporter NarU -
JR901_RS23510 67011..67991 + 981 WP_205899142.1 IS5 family transposase -
JR901_RS23515 68139..68420 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JR901_RS23520 68410..68661 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
JR901_RS23525 69821..70789 + 969 WP_000654812.1 IS5-like element IS903B family transposase -
JR901_RS23530 70949..71092 + 144 WP_152930453.1 hypothetical protein -
JR901_RS23535 71153..71401 + 249 Protein_80 transposase -
JR901_RS23540 71466..72617 + 1152 WP_001254932.1 IS30-like element IS30 family transposase -
JR901_RS23545 72630..72887 - 258 Protein_82 transposase -
JR901_RS23550 72962..73273 + 312 Protein_83 VRR-NUC domain-containing protein -
JR901_RS23555 73340..73495 + 156 WP_032236586.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / sul3 / ant(3'')-Ia / floR / tet(A) - 1..142545 142545
- inside IScluster/Tn - - 67011..72887 5876


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T194638 WP_000220560.1 NZ_CP070907:c68420-68139 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T194638 NZ_CP093913:c319521-319231 [Aggregatibacter actinomycetemcomitans]
ATGACTTATAAACTCACTTTTGATAAAAGGGCTTTAAAAGAGTGGGAAAAGTTAGGTGACACCATACGCCAACAATTCAA
AAATAAACTTGCGGAACGCTTAGAAAATCCGAGAGTACCCGGCGACAAATTAAAAGGTTATCAAAACCTTTATAAAATCA
AACTCCGCGCAGCCGGTTATCGCTTAGTTTATGAAGTTAATGATAACCAAATTTATATTCTTGTTTTATCCGTGGGTAAA
CGGAATAGATTAAATGCATACAAAAATGCAGAATTCAGACAAGCCCGGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT194638 WP_000121743.1 NZ_CP070907:c68661-68410 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT194638 NZ_CP093913:c319765-319511 [Aggregatibacter actinomycetemcomitans]
ATGGCAATACATCAAATTTTGACACAACAAGCCGCAAGCATTACCGATTTAAAACGGGACCCAATGGGCATTGCGTACGC
AAGCCAAGATGGTGCCGTAGCCATATTAAACCGTAACCAACCGGCCTTTTATTGCGTCACGCCTGCATTATTTGAATATT
TTCAAAATCTGGTAGAAGATGCAGAATTAGGCAGAATCGCTGAAGAGCGTCTGGCGACCCTTGATCCGATTGAGGTAAGC
CTTGATGACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References