Detailed information of TA system TA194635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4279046..4279596
• Replicon: chromosome
• Accession: NZ_CP070906
• Organism: Escherichia coli strain 88COLEC

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: JR901_RS20300
• Protein ID: WP_001160975.1
• Coordinates: 4279282..4279596 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: D3H431
• Locus tag: JR901_RS20295
• Protein ID: WP_000125565.1
• Coordinates: 4279046..4279279 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR901_RS20270	4274117..4274404	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
JR901_RS20275	4274462..4275793	+	1332	WP_001183198.1	MFS transporter	-
JR901_RS20280	4275904..4276434	+	531	WP_000600718.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
JR901_RS20285	4276427..4278289	+	1863	WP_040234481.1	glutathione-regulated potassium-efflux system protein KefC	-
JR901_RS20290	4278481..4278960	+	480	WP_205899076.1	type 3 dihydrofolate reductase	-
JR901_RS20295	4279046..4279279	+	234	WP_000125565.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JR901_RS20300	4279282..4279596	+	315	WP_001160975.1	CcdB family protein	Toxin
JR901_RS20305	4279593..4280441	-	849	WP_021556397.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
JR901_RS20310	4280448..4280825	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
JR901_RS20315	4280828..4281649	-	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
JR901_RS20320	4281646..4282635	-	990	WP_021556398.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
JR901_RS20325	4282635..4283921	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T194635 WP_001160975.1 NZ_CP070906:4279282-4279596 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T194635 NZ_CP093912:991198-991488 [Aggregatibacter actinomycetemcomitans]
ATGACTTATAAACTCACTTTTGATAAAAGGGCTTTAAAAGAGTGGCAAAAGTTAGGCGATACCATACGCCAACAATTCAA
AAATAAACTTGCGGAACGCTTAGAAAATCCGAGAGTACCGGGCGACAAATTAAGAGGTTATCAAAACCTTTATAAAATCA
AACTCCGCGCAGCCGGTTATCGCTTAGTTTATGAAGTTAATGATAACCAAATTTATATTCTTGTTTTATCCGTGGGTAAA
CGGAATAGATTAGACGCATATAAAAACGCAGAATTCAGACAAGCTCGGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8599.64 Da
• Isoelectric Point: 6.9791

>AT194635 WP_000125565.1 NZ_CP070906:4279046-4279279 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRRF

Nucleotide

• Length: 234 bp

>AT194635 NZ_CP093912:990954-991208 [Aggregatibacter actinomycetemcomitans]
ATGGCAATACATCAAATTTTGACACAACAAGCCGCAAGCATTACCGATTTAAAACGGGACCCAATGGGCATTGCGTACGC
AAGCCAAGATGGTGCCGTAGCCATATTAAACCATAACCAACCAGCCTTTTATTGCGTCACGCCTGCATTATTTGAATATT
TTCAAAATCTGGTAGAAGATGCAGAATTAGGCAGAATTGCTGAAGAGCGTCTGGCAACCCTTGATCCGATTGAGGTAAGC
CTTGATGACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A090NGC7