Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3564253..3564871 Replicon chromosome
Accession NZ_CP070906
Organism Escherichia coli strain 88COLEC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JR901_RS16930 Protein ID WP_001291435.1
Coordinates 3564653..3564871 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JR901_RS16925 Protein ID WP_000344800.1
Coordinates 3564253..3564627 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR901_RS16915 3559781..3562930 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
JR901_RS16920 3563259..3563956 + 698 Protein_3311 IS1 family transposase -
JR901_RS16925 3564253..3564627 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JR901_RS16930 3564653..3564871 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JR901_RS16935 3565042..3565593 + 552 WP_000102564.1 maltose O-acetyltransferase -
JR901_RS16940 3565709..3566179 + 471 WP_000136192.1 YlaC family protein -
JR901_RS16945 3566343..3567893 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JR901_RS16950 3567935..3568288 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JR901_RS16960 3568667..3568978 + 312 WP_000409911.1 MGMT family protein -
JR901_RS16965 3569009..3569581 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T194632 WP_001291435.1 NZ_CP070906:3564653-3564871 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T194632 NZ_CP093912:c681703-681401 [Aggregatibacter actinomycetemcomitans]
ATGTATATAATTGAGGAAACGGAGGAATTCTCCAACTGGTTAGAGAATCTAACCGACGAACTTGCGATTGTGAATATTGT
TGCTCGAATCGAAAGGGCAAAGCTCGGCAATTTCGGTGATCATAAATCGGTGGGTAATGGCGTATATGAAATGCGAATTA
CAAAAGGGAAAGGCTATAGGATCTACTATGCCAGAAAAGGGGGTATTACTTATCTATTGATTCTTGGTGGCGATAAGTCA
AGCCAAGAACAAGATATTCAGAATGCAAAAGCACTATGGCAACAGATTAAAAACGGAGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT194632 WP_000344800.1 NZ_CP070906:3564253-3564627 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT194632 NZ_CP093912:c681399-681106 [Aggregatibacter actinomycetemcomitans]
ATGACAGTAACAACCCGAGAATTTGATGCAGCGCGTTATTTGAAAAATGAAAAAATGATGGCAATGTATCTTGAAACAGT
TTTAGAAGAAGGCACAACGGCTGAGTTTATACAAGCTCTGAATACGGTCGCAAGAGCAAAGGGCATGTCGGAACTGGCGG
AAAAAACCGGCATCGGACGTGAAAGTTTGTACAAAACCCTATCAAGTGAAAAACCACGTTTTGACACGATTCTAAAAATT
ATTTCAGCATTGGGGCTTAGCCTTTCTATTAATGCTAAAGATGCAGTAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References