Detailed information of TA system TA194631

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2865585..2866420
• Replicon: chromosome
• Accession: NZ_CP070906
• Organism: Escherichia coli strain 88COLEC

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A6H2GFM1
• Locus tag: JR901_RS13780
• Protein ID: WP_065304416.1
• Coordinates: 2865585..2865962 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JR901_RS13785
• Protein ID: WP_142764154.1
• Coordinates: 2866052..2866420 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR901_RS13740	2860855..2861346	-	492	WP_205899138.1	DUF1097 family protein	-
JR901_RS13745	2861448..2862002	-	555	WP_021556694.1	molecular chaperone YcdY	-
JR901_RS13750	2862026..2862763	-	738	WP_000283664.1	zinc-binding phosphatase	-
JR901_RS13755	2862818..2863756	-	939	WP_040234658.1	glyoxylate/hydroxypyruvate reductase GhrA	-
JR901_RS13765	2864227..2865069	-	843	WP_065304417.1	DUF4942 domain-containing protein	-
JR901_RS13770	2865166..2865363	-	198	WP_086598277.1	DUF957 domain-containing protein	-
JR901_RS13775	2865409..2865588	-	180	Protein_2700	hypothetical protein	-
JR901_RS13780	2865585..2865962	-	378	WP_065304416.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JR901_RS13785	2866052..2866420	-	369	WP_142764154.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JR901_RS13790	2866470..2867114	-	645	WP_205899050.1	antitoxin of toxin-antitoxin stability system	-
JR901_RS13795	2867129..2867350	-	222	WP_000692312.1	DUF987 domain-containing protein	-
JR901_RS13800	2867419..2867895	-	477	WP_001186742.1	RadC family protein	-
JR901_RS13805	2867910..2868395	-	486	WP_000214316.1	antirestriction protein	-
JR901_RS13810	2868486..2869304	-	819	WP_205899051.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14060.06 Da
• Isoelectric Point: 7.8522

>T194631 WP_065304416.1 NZ_CP070906:c2865962-2865585 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKQ

Nucleotide

• Length: 378 bp

>T194631 NZ_CP093912:c681399-681106 [Aggregatibacter actinomycetemcomitans]
ATGACAGTAACAACCCGAGAATTTGATGCAGCGCGTTATTTGAAAAATGAAAAAATGATGGCAATGTATCTTGAAACAGT
TTTAGAAGAAGGCACAACGGCTGAGTTTATACAAGCTCTGAATACGGTCGCAAGAGCAAAGGGCATGTCGGAACTGGCGG
AAAAAACCGGCATCGGACGTGAAAGTTTGTACAAAACCCTATCAAGTGAAAAACCACGTTTTGACACGATTCTAAAAATT
ATTTCAGCATTGGGGCTTAGCCTTTCTATTAATGCTAAAGATGCAGTAGCCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13825.55 Da
• Isoelectric Point: 5.9618

>AT194631 WP_142764154.1 NZ_CP070906:c2866420-2866052 [Escherichia coli]
VSDTFHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMALYPTPETKK

Nucleotide

• Length: 369 bp

>AT194631 NZ_CP093912:c681703-681401 [Aggregatibacter actinomycetemcomitans]
ATGTATATAATTGAGGAAACGGAGGAATTCTCCAACTGGTTAGAGAATCTAACCGACGAACTTGCGATTGTGAATATTGT
TGCTCGAATCGAAAGGGCAAAGCTCGGCAATTTCGGTGATCATAAATCGGTGGGTAATGGCGTATATGAAATGCGAATTA
CAAAAGGGAAAGGCTATAGGATCTACTATGCCAGAAAAGGGGGTATTACTTATCTATTGATTCTTGGTGGCGATAAGTCA
AGCCAAGAACAAGATATTCAGAATGCAAAAGCACTATGGCAACAGATTAAAAACGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A6H2GFM1