Detailed information of TA system TA194630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2366062..2367650
• Replicon: chromosome
• Accession: NZ_CP070906
• Organism: Escherichia coli strain 88COLEC

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: F4NFZ0
• Locus tag: JR901_RS11300
• Protein ID: WP_001125463.1
• Coordinates: 2366328..2367650 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JR901_RS11295
• Protein ID: WP_001296726.1
• Coordinates: 2366062..2366328 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR901_RS11290	2364456..2365853	+	1398	WP_124848636.1	autotransporter outer membrane beta-barrel domain-containing protein	-
JR901_RS11295	2366062..2366328	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JR901_RS11300	2366328..2367650	+	1323	WP_001125463.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JR901_RS11305	2367962..2368176	+	215	Protein_2210	helix-turn-helix transcriptional regulator	-
JR901_RS11310	2368170..2368867	-	698	Protein_2211	IS1 family transposase	-
JR901_RS11315	2368954..2369628	+	675	WP_001323842.1	fimbria/pilus periplasmic chaperone	-
JR901_RS11320	2369670..2372321	+	2652	WP_040234717.1	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2366062..2384432	18370

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49422.05 Da
• Isoelectric Point: 9.1203

>T194630 WP_001125463.1 NZ_CP070906:2366328-2367650 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T194630 NZ_CP093912:575948-576298 [Aggregatibacter actinomycetemcomitans]
ATGTGGGCGGTGATTTTAACGGAACGATTTGAAACTTGGTTAAATCAACAGTCGATAAAAACGCAGGAATGTGTTTTGGC
AAACTTGGTACGGTTGGAACATTTTGGTCCGAATTTAGCCCGTCCTTATGTAGATAGCGTAGCAGGGTCAATTCATAGCA
ACATGAAAGAACTACGCGTTCAGCATTCGGGAAGAGCAATTCGACTCTTTTTTGCTTTTGATCCAAAGCGTAGAGCCATT
GTTTTATGTGGTGGAGATAAAGGCAATGACAAGCGGTTTTACCGTACGATGATTAGAATTGCAGATCAAGAATTGAATCA
TTATTTGGCAACCATGGAGCAAACAAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT194630 WP_001296726.1 NZ_CP070906:2366062-2366328 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT194630 NZ_CP093912:576295-576594 [Aggregatibacter actinomycetemcomitans]
ATGAAAACATTACAACAAGTTATTGATGAACGTTCGGTAGAAAGCCGCCAACGTATTCAAACCATGGCGGAAGATTTAAT
TCTTGAAACCGGTTTGCAACTGTTGCGAGAAGAATTAAATATATCGCAACAACAATTAGCTACGATTTTAGGTGTAACTC
AACCTGCAATTAATCAACTGGAACAGCGTGGCAATGAAATTAAGCTTGCAACCCTCAAACGTTACATCGAAGCAATGGGC
GGTAAATTACGCCTTAGTGTAGAAATGCCTGATGGACATCAGCGGGTTTTTCAAATTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9IYX0