Detailed information of TA system TA194617

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 822103..822901
• Replicon: chromosome
• Accession: NZ_CP070906
• Organism: Escherichia coli strain 88COLEC

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: U9XMP3
• Locus tag: JR901_RS03905
• Protein ID: WP_000854735.1
• Coordinates: 822103..822480 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A067H947
• Locus tag: JR901_RS03910
• Protein ID: WP_001285415.1
• Coordinates: 822527..822901 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR901_RS03870	817669..818847	+	1179	WP_021557196.1	type II secretion system protein GspL	-
JR901_RS03875	818849..819385	+	537	WP_000942790.1	GspM family type II secretion system protein YghD	-
JR901_RS03880	819800..820126	-	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JR901_RS03885	820123..820386	-	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
JR901_RS03890	820458..821324	-	867	WP_001280433.1	DUF4942 domain-containing protein	-
JR901_RS03895	821409..821606	-	198	WP_061355066.1	DUF957 domain-containing protein	-
JR901_RS03900	821618..822106	-	489	WP_205899091.1	hypothetical protein	-
JR901_RS03905	822103..822480	-	378	WP_000854735.1	TA system toxin CbtA family protein	Toxin
JR901_RS03910	822527..822901	-	375	WP_001285415.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JR901_RS03915	822981..823202	-	222	WP_000692309.1	DUF987 domain-containing protein	-
JR901_RS03920	823265..823741	-	477	WP_001366855.1	RadC family protein	-
JR901_RS03925	823757..824236	-	480	WP_000844100.1	antirestriction protein	-
JR901_RS03930	824318..825136	-	819	WP_001175148.1	DUF945 domain-containing protein	-
JR901_RS03935	825226..825459	-	234	WP_001278287.1	DUF905 family protein	-
JR901_RS03940	825465..826142	-	678	WP_001097301.1	hypothetical protein	-
JR901_RS03945	826290..826970	-	681	WP_001282921.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	820123..854379	34256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14061.07 Da
• Isoelectric Point: 8.2904

>T194617 WP_000854735.1 NZ_CP070906:c822480-822103 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T194617 NZ_CP093911:1317572-1317838 [Aggregatibacter actinomycetemcomitans]
ATGGATTACGTTTTATCAAAAGAATATAAACGGGACTTAAAAAAACTTCCGGTCGAAATTCAATCCGGTCCGGAATATGC
AGAGGTTTTATATTGCCTGTTCAATCAAAAATCCCTACCCGAACGATATAAAGATCATGCTTTGCAAGGAAATTGGCAGG
GATTTCGAGATTGCCACATCAAAAATGATCTCATTTTAATTTACAAAATAGAAGCAGACACCCTCTATTTTGCAAGGCTA
AATTCTCACTCGGAAGTGTTTAAATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13663.32 Da
• Isoelectric Point: 5.8640

>AT194617 WP_001285415.1 NZ_CP070906:c822901-822527 [Escherichia coli]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT194617 NZ_CP093911:1317288-1317560 [Aggregatibacter actinomycetemcomitans]
ATGTTAGATAGCGCAGTGAATTTCCGTACTCAAGCCGACATCAAAGAGCAAGCGTTTAACGTCATTAAAAGCTATGGCTT
AACGCCCGCTCAGGTGTTAAATATGTTTTTGACCCAAATTGCCAAAACCAACACAATTCCGCTCAGTTTGGATTATCAGC
CGAATACAAAAACAGCTAATGCAATCAATGAATTAATGAGCGGAAAAGGGGAAAGATTTAGCGTAGATTCCTTTGATGAA
TTTCAACAAAAAATGCGTGATTTAAGCAAATAA

Structures

Toxin

• AlphaFold DB: U9XMP3

Antitoxin

• AlphaFold DB: A0A067H947