Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5880..6405 Replicon plasmid pJNQH116-2
Accession NZ_CP070900
Organism Klebsiella pneumoniae strain JNQH116

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag JYA99_RS26535 Protein ID WP_013023785.1
Coordinates 5880..6185 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag JYA99_RS26540 Protein ID WP_001568025.1
Coordinates 6187..6405 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JYA99_RS26505 1559..2185 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
JYA99_RS26510 2305..2484 + 180 WP_006788217.1 hypothetical protein -
JYA99_RS26515 2956..3750 - 795 WP_004197635.1 site-specific integrase -
JYA99_RS26520 3948..4964 - 1017 WP_017899884.1 hypothetical protein -
JYA99_RS26525 4975..5289 - 315 WP_053389906.1 hypothetical protein -
JYA99_RS26530 5316..5711 - 396 WP_017899885.1 hypothetical protein -
JYA99_RS26535 5880..6185 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
JYA99_RS26540 6187..6405 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JYA99_RS26545 7223..7513 - 291 WP_013023783.1 hypothetical protein -
JYA99_RS26550 7510..8637 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
JYA99_RS26555 8671..10263 - 1593 WP_015344964.1 hypothetical protein -
JYA99_RS26560 10470..11249 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / blaNDM-1 / tet(A) / blaCTX-M-15 / blaTEM-1C htpB 1..136763 136763


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T194593 WP_013023785.1 NZ_CP070900:c6185-5880 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T194593 NZ_CP093856:2680998-2681366 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGTCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT194593 WP_001568025.1 NZ_CP070900:c6405-6187 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT194593 NZ_CP093856:2680780-2681001 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References