Detailed information of TA system TA194592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 125606..126276
• Replicon: plasmid pJNQH116-1
• Accession: NZ_CP070899
• Organism: Klebsiella pneumoniae strain JNQH116

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: JYA99_RS25965
• Protein ID: WP_004213072.1
• Coordinates: 125606..126049 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: JYA99_RS25970
• Protein ID: WP_004213073.1
• Coordinates: 126046..126276 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYA99_RS25930	121025..121300	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
JYA99_RS25935	121363..121854	+	492	WP_011251327.1	DM13 domain-containing protein	-
JYA99_RS25940	121903..122823	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
JYA99_RS25945	122914..123320	+	407	Protein_125	GAF domain-containing protein	-
JYA99_RS25950	123834..124469	-	636	WP_165452888.1	response regulator	-
JYA99_RS25955	124879..125172	+	294	Protein_127	transposase	-
JYA99_RS25960	125176..125592	+	417	Protein_128	restriction endonuclease	-
JYA99_RS25965	125606..126049	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
JYA99_RS25970	126046..126276	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JYA99_RS25975	126884..128017	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
JYA99_RS25980	128033..128326	+	294	WP_004213076.1	hypothetical protein	-
JYA99_RS25985	128316..128522	-	207	WP_004213077.1	hypothetical protein	-
JYA99_RS25990	128874..129164	+	291	WP_004213078.1	hypothetical protein	-
JYA99_RS25995	129154..130053	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA / pla	1..225276	225276

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T194592 WP_004213072.1 NZ_CP070899:c126049-125606 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T194592 NZ_CP093856:c1450695-1450285 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT194592 WP_004213073.1 NZ_CP070899:c126276-126046 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT194592 NZ_CP093856:c1450942-1450676 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620