Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5189230..5189855 Replicon chromosome
Accession NZ_CP070898
Organism Klebsiella pneumoniae strain JNQH116

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JYA99_RS24960 Protein ID WP_002882817.1
Coordinates 5189230..5189613 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JYA99_RS24965 Protein ID WP_004150355.1
Coordinates 5189613..5189855 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JYA99_RS24945 5186596..5187498 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JYA99_RS24950 5187495..5188130 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JYA99_RS24955 5188127..5189056 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JYA99_RS24960 5189230..5189613 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JYA99_RS24965 5189613..5189855 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JYA99_RS24970 5190060..5190977 + 918 WP_012737090.1 alpha/beta hydrolase -
JYA99_RS24975 5190991..5191932 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
JYA99_RS24980 5191977..5192414 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JYA99_RS24985 5192411..5193271 - 861 WP_004146232.1 virulence factor BrkB family protein -
JYA99_RS24990 5193265..5193864 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T194589 WP_002882817.1 NZ_CP070898:c5189613-5189230 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T194589 NZ_CP093855:151727-152011 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT194589 WP_004150355.1 NZ_CP070898:c5189855-5189613 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT194589 NZ_CP093855:151489-151737 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References