Detailed information of TA system TA194585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3987531..3988150
• Replicon: chromosome
• Accession: NZ_CP070898
• Organism: Klebsiella pneumoniae strain JNQH116

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: JYA99_RS19275
• Protein ID: WP_002892050.1
• Coordinates: 3987932..3988150 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: J2DPF6
• Locus tag: JYA99_RS19270
• Protein ID: WP_002892066.1
• Coordinates: 3987531..3987905 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYA99_RS19260	3982683..3983876	+	1194	WP_002892072.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
JYA99_RS19265	3983899..3987045	+	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
JYA99_RS19270	3987531..3987905	+	375	WP_002892066.1	Hha toxicity modulator TomB	Antitoxin
JYA99_RS19275	3987932..3988150	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
JYA99_RS19280	3988309..3988875	+	567	WP_002892030.1	maltose O-acetyltransferase	-
JYA99_RS19285	3988847..3988987	-	141	WP_004147370.1	hypothetical protein	-
JYA99_RS19290	3989008..3989478	+	471	WP_002892026.1	YlaC family protein	-
JYA99_RS19295	3989453..3990904	-	1452	WP_002892023.1	PLP-dependent aminotransferase family protein	-
JYA99_RS19300	3991005..3991703	+	699	WP_032414604.1	GNAT family N-acetyltransferase	-
JYA99_RS19305	3991700..3991840	-	141	WP_002892018.1	type B 50S ribosomal protein L36	-
JYA99_RS19310	3991840..3992103	-	264	WP_002892011.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T194585 WP_002892050.1 NZ_CP070898:3987932-3988150 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T194585 NZ_CP093854:5685791-5686261 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14368.06 Da
• Isoelectric Point: 4.8989

>AT194585 WP_002892066.1 NZ_CP070898:3987531-3987905 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT194585 NZ_CP093854:5685351-5685794 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2

Antitoxin

• AlphaFold DB: A0A0H3GJ93