Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 24277..24920 Replicon plasmid pKP-135LU_R
Accession NZ_CP070893
Organism Klebsiella pneumoniae strain KP-135LU

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag HZC98_RS28725 Protein ID WP_000754566.1
Coordinates 24277..24693 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HZC98_RS28730 Protein ID WP_001261276.1
Coordinates 24690..24920 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZC98_RS28690 (HZC98_28580) 19551..19661 + 111 Protein_23 hypothetical protein -
HZC98_RS28695 (HZC98_28585) 19658..20269 - 612 WP_000176305.1 DUF2913 family protein -
HZC98_RS28700 (HZC98_28590) 20323..20604 - 282 WP_001568067.1 hypothetical protein -
HZC98_RS28705 (HZC98_28595) 20786..21112 + 327 WP_004201125.1 C2H2-type zinc finger protein -
HZC98_RS28710 (HZC98_28600) 21738..21839 + 102 Protein_27 Tn3 family transposase -
HZC98_RS28715 (HZC98_28605) 21859..22935 - 1077 WP_000227969.1 IS110 family transposase -
HZC98_RS28720 (HZC98_28610) 23217..24073 - 857 Protein_29 IS3-like element ISEc15 family transposase -
HZC98_RS28725 (HZC98_28615) 24277..24693 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
HZC98_RS28730 (HZC98_28620) 24690..24920 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZC98_RS28735 (HZC98_28625) 25494..25844 + 351 WP_000493378.1 hypothetical protein -
HZC98_RS28740 (HZC98_28630) 25895..26638 + 744 WP_000129823.1 hypothetical protein -
HZC98_RS28745 (HZC98_28635) 26635..27411 + 777 WP_000015958.1 site-specific integrase -
HZC98_RS28750 (HZC98_28640) 27469..27726 - 258 WP_000764642.1 hypothetical protein -
HZC98_RS28755 (HZC98_28645) 27855..27959 - 105 WP_032409716.1 hypothetical protein -
HZC98_RS28760 (HZC98_28650) 28489..29355 + 867 WP_004118283.1 replication initiation protein -
HZC98_RS28765 (HZC98_28655) 29532..29801 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / qnrS1 / aph(3')-VI / blaNDM-1 / aac(6')-Ib-cr / ARR-3 / qacE - 1..42041 42041
- inside Integron - - 358..42040 41682


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T194570 WP_000754566.1 NZ_CP070893:c24693-24277 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T194570 NZ_CP093853:c122530-122048 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGATGTCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGAAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT194570 WP_001261276.1 NZ_CP070893:c24920-24690 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT194570 NZ_CP093853:c122799-122521 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACACCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References