Detailed information of TA system TA194569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-DnaT
• Location: 260899..261421
• Replicon: plasmid pKP-135LU_HIB-FIB
• Accession: NZ_CP070891
• Organism: Klebsiella pneumoniae strain KP-135LU

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: HZC98_RS27605
• Protein ID: WP_004181778.1
• Coordinates: 261137..261421 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A5Q9LM53
• Locus tag: HZC98_RS27600
• Protein ID: WP_025368601.1
• Coordinates: 260899..261147 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZC98_RS27580 (HZC98_27490)	256108..256929	-	822	WP_004026535.1	hypothetical protein	-
HZC98_RS27585 (HZC98_27495)	256991..257344	-	354	WP_004181774.1	hypothetical protein	-
HZC98_RS27590 (HZC98_27500)	257489..258475	-	987	WP_025368599.1	hypothetical protein	-
HZC98_RS27595 (HZC98_27505)	258809..260608	-	1800	WP_025368600.1	ATP-dependent helicase	-
HZC98_RS27600 (HZC98_27510)	260899..261147	+	249	WP_025368601.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HZC98_RS27605 (HZC98_27515)	261137..261421	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZC98_RS27610 (HZC98_27520)	261651..262877	+	1227	WP_025368602.1	RNA-guided endonuclease TnpB family protein	-
HZC98_RS27615 (HZC98_27525)	263163..263588	-	426	WP_040120489.1	IS200/IS605 family transposase	-
HZC98_RS27620 (HZC98_27530)	263625..264851	+	1227	WP_025368604.1	RNA-guided endonuclease TnpB family protein	-
HZC98_RS27625 (HZC98_27535)	264917..266043	-	1127	Protein_288	conjugal transfer protein TraG N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / qacE / dfrA5 / mph(E) / msr(E) / armA / sul2 / blaCTX-M-15	rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..341276	341276

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T194569 WP_004181778.1 NZ_CP070891:261137-261421 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T194569 NZ_CP093853:c76474-76031 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8906.12 Da
• Isoelectric Point: 3.8206

>AT194569 WP_025368601.1 NZ_CP070891:260899-261147 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGHIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT194569 NZ_CP093853:c76701-76471 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A5Q9LM53