Detailed information of TA system TA194568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 156516..157186
• Replicon: plasmid pKP-135LU_HIB-FIB
• Accession: NZ_CP070891
• Organism: Klebsiella pneumoniae strain KP-135LU

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: HZC98_RS27065
• Protein ID: WP_004213072.1
• Coordinates: 156516..156959 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: HZC98_RS27070
• Protein ID: WP_004213073.1
• Coordinates: 156956..157186 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZC98_RS27030 (HZC98_26935)	151923..152198	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
HZC98_RS27035 (HZC98_26940)	152261..152752	+	492	WP_011251327.1	DM13 domain-containing protein	-
HZC98_RS27040 (HZC98_26945)	152801..153721	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
HZC98_RS27045 (HZC98_26950)	153812..154215	+	404	Protein_172	GAF domain-containing protein	-
HZC98_RS27050 (HZC98_26955)	154734..155372	-	639	Protein_173	mucoid phenotype regulator RmpA2	-
HZC98_RS27055 (HZC98_26960)	155789..156093	+	305	Protein_174	transposase	-
HZC98_RS27060	156116..156367	-	252	WP_186987481.1	hypothetical protein	-
HZC98_RS27065 (HZC98_26970)	156516..156959	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HZC98_RS27070 (HZC98_26975)	156956..157186	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HZC98_RS27075 (HZC98_26980)	157794..158927	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
HZC98_RS27080 (HZC98_26985)	158943..159236	+	294	WP_004213076.1	hypothetical protein	-
HZC98_RS27085 (HZC98_26990)	159226..159432	-	207	WP_004213077.1	hypothetical protein	-
HZC98_RS27090 (HZC98_26995)	159784..160074	+	291	WP_004213078.1	hypothetical protein	-
HZC98_RS27095 (HZC98_27000)	160064..160963	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / qacE / dfrA5 / mph(E) / msr(E) / armA / sul2 / blaCTX-M-15	rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..341276	341276

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T194568 WP_004213072.1 NZ_CP070891:c156959-156516 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T194568 NZ_CP093853:2339-2809 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT194568 WP_004213073.1 NZ_CP070891:c157186-156956 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT194568 NZ_CP093853:1899-2342 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620