Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 38560..39470 Replicon plasmid pKP-135LU_HIB-FIB
Accession NZ_CP070891
Organism Klebsiella pneumoniae strain KP-135LU

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag HZC98_RS26440 Protein ID WP_004026354.1
Coordinates 39000..39470 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag HZC98_RS26435 Protein ID WP_004181895.1
Coordinates 38560..39003 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZC98_RS26420 (HZC98_26340) 35193..36680 + 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
HZC98_RS26425 (HZC98_26345) 36766..37188 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
HZC98_RS26430 (HZC98_26350) 37188..38459 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
HZC98_RS26435 (HZC98_26355) 38560..39003 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
HZC98_RS26440 (HZC98_26360) 39000..39470 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
HZC98_RS26445 (HZC98_26365) 39580..39840 - 261 WP_004026352.1 hypothetical protein -
HZC98_RS26450 (HZC98_26370) 40562..40720 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
HZC98_RS26455 (HZC98_26375) 41323..42297 + 975 WP_058842863.1 hypothetical protein -
HZC98_RS28935 42281..42403 + 123 WP_257183836.1 hypothetical protein -
HZC98_RS26460 (HZC98_26380) 42495..42872 - 378 WP_029884711.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / sul1 / qacE / dfrA5 / mph(E) / msr(E) / armA / sul2 / blaCTX-M-15 rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..341276 341276
- flank IS/Tn - - 33528..34748 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T194567 WP_004026354.1 NZ_CP070891:39000-39470 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T194567 NZ_CP093852:c5684115-5683645 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT194567 WP_004181895.1 NZ_CP070891:38560-39003 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT194567 NZ_CP093852:c5684555-5684112 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References