Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5285085..5285710 Replicon chromosome
Accession NZ_CP070890
Organism Klebsiella pneumoniae strain KP-135LU

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HZC98_RS25765 Protein ID WP_002882817.1
Coordinates 5285085..5285468 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HZC98_RS25770 Protein ID WP_004150355.1
Coordinates 5285468..5285710 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZC98_RS25750 (5282451) 5282451..5283353 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HZC98_RS25755 (5283350) 5283350..5283985 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HZC98_RS25760 (5283982) 5283982..5284911 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HZC98_RS25765 (5285085) 5285085..5285468 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HZC98_RS25770 (5285468) 5285468..5285710 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HZC98_RS25775 (5285915) 5285915..5286832 + 918 WP_021466676.1 alpha/beta hydrolase -
HZC98_RS25780 (5286846) 5286846..5287787 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HZC98_RS25785 (5287832) 5287832..5288269 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HZC98_RS25790 (5288266) 5288266..5289126 - 861 WP_004146232.1 virulence factor BrkB family protein -
HZC98_RS25795 (5289120) 5289120..5289719 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T194566 WP_002882817.1 NZ_CP070890:c5285468-5285085 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T194566 NZ_CP093852:c5571191-5570658 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT194566 WP_004150355.1 NZ_CP070890:c5285710-5285468 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT194566 NZ_CP093852:c5571468-5571202 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References