Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1469646..1470382 Replicon chromosome
Accession NZ_CP070890
Organism Klebsiella pneumoniae strain KP-135LU

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag HZC98_RS07260 Protein ID WP_032433360.1
Coordinates 1469900..1470382 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HZC98_RS07255 Protein ID WP_003026799.1
Coordinates 1469646..1469912 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZC98_RS07230 (1465292) 1465292..1466431 + 1140 WP_032433368.1 mannitol dehydrogenase -
HZC98_RS07235 (1466460) 1466460..1467122 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
HZC98_RS07240 (1467103) 1467103..1468110 + 1008 WP_280954017.1 dihydroxyacetone kinase subunit DhaK -
HZC98_RS07245 (1468128) 1468128..1468760 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
HZC98_RS07250 (1468770) 1468770..1469333 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
HZC98_RS07255 (1469646) 1469646..1469912 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HZC98_RS07260 (1469900) 1469900..1470382 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
HZC98_RS07265 (1470582) 1470582..1471985 + 1404 WP_001272054.1 ISNCY-like element ISKpn21 family transposase -
HZC98_RS07270 (1472014) 1472014..1472646 - 633 WP_001567369.1 hypothetical protein -
HZC98_RS07275 (1473026) 1473026..1473625 - 600 WP_064472304.1 helix-turn-helix transcriptional regulator -
HZC98_RS07280 (1473838) 1473838..1474782 - 945 WP_077254249.1 fimbrial protein -
HZC98_RS07285 (1474794) 1474794..1475372 - 579 WP_032432061.1 type 1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1437591..1483608 46017
- flank IS/Tn - - 1470582..1471985 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T194552 WP_032433360.1 NZ_CP070890:1469900-1470382 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T194552 NZ_CP093551:c5016-4741 [Escherichia coli]
ATGACACGCTACCGGACAGAATACACAAAACGGTTCGTCAAAGAGTTTAAGAAAATTGACACGGTACACCAGAGGGTAAT
TCAGAAGTACATTGAGGCGCACATCAACAACGCACTCAATCCGCGCTTGACGGGTAAAATCCTGAAAGGAGATAAAGCCG
AATTTATCCGCTGGCGTATCGGTGATTACCGTCTGATTGCCACTATTGAAGACGATAAATTGCTGGTCATTGCGTTAAGC
GTGGGACACCGCAAAGACGTGTACAAAAAGTTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT194552 WP_003026799.1 NZ_CP070890:1469646-1469912 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT194552 NZ_CP093551:c5252-5028 [Escherichia coli]
ATGAACAAAAACATGACGAAAGCGATCCGGTTGAATGACGAAGATTTTAACCTGTTTGAGTCTTACGCCAACGCAAAAGG
GATAACCTTCAGCGAATTGTGCAAAAGCGCAGTACGGGAAAAAATCGAGGACGAATTAGATTTGCAATGTGCTAATGAAA
GCTACGCGGATTATCTCAGCGACAAAACGACAATCAGCCACGACGAACTGTTTAAATCTATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References