Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 76565..77166 Replicon plasmid pTB91P2
Accession NZ_CP070875
Organism Escherichia fergusonii strain EF91

Toxin (Protein)


Gene name doc Uniprot ID F0JYA5
Locus tag JW972_RS22955 Protein ID WP_001216044.1
Coordinates 76565..76945 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag JW972_RS22960 Protein ID WP_001190712.1
Coordinates 76945..77166 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW972_RS22925 (JW972_22770) 72543..72764 - 222 WP_000542336.1 hypothetical protein -
JW972_RS22935 (JW972_22780) 74138..74347 + 210 WP_000874154.1 hypothetical protein -
JW972_RS22940 (JW972_22785) 74458..75309 + 852 WP_000611653.1 phage repressor protein C1 -
JW972_RS23385 75342..75467 - 126 WP_257233066.1 hypothetical protein -
JW972_RS22945 (JW972_22790) 75484..76353 - 870 Protein_92 DUF968 domain-containing protein -
JW972_RS22950 (JW972_22795) 76381..76560 - 180 WP_039022005.1 hypothetical protein -
JW972_RS22955 (JW972_22800) 76565..76945 - 381 WP_001216044.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JW972_RS22960 (JW972_22805) 76945..77166 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JW972_RS22965 (JW972_22810) 77551..78527 - 977 Protein_96 IS5 family transposase -
JW972_RS22970 (JW972_22815) 78788..80175 + 1388 Protein_97 nitrate/nitrite transporter NarU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / qnrS1 - 1..128710 128710
- flank IS/Tn - - 77997..78527 530


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13544.28 Da        Isoelectric Point: 5.6343

>T194547 WP_001216044.1 NZ_CP070875:c76945-76565 [Escherichia fergusonii]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Download         Length: 381 bp

>T194547 NZ_CP093548:c5031323-5030937 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT194547 WP_001190712.1 NZ_CP070875:c77166-76945 [Escherichia fergusonii]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT194547 NZ_CP093548:c5030947-5030618 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6Y6HMN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References