Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 78804..80427 Replicon plasmid pTB91P1
Accession NZ_CP070874
Organism Escherichia fergusonii strain EF91

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag JW972_RS21425 Protein ID WP_000088046.1
Coordinates 79102..80427 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag JW972_RS21420 Protein ID WP_024136327.1
Coordinates 78804..79097 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW972_RS21395 (JW972_21335) 74676..75917 + 1242 WP_001053338.1 VWA domain-containing protein -
JW972_RS23355 76008..76139 + 132 WP_000374059.1 hypothetical protein -
JW972_RS21400 (JW972_21340) 76480..76761 - 282 WP_223667018.1 hypothetical protein -
JW972_RS21405 (JW972_21345) 76811..77002 - 192 WP_000398480.1 hypothetical protein -
JW972_RS21410 (JW972_21350) 77094..77435 - 342 WP_001151575.1 hypothetical protein -
JW972_RS21415 (JW972_21355) 77808..78200 + 393 WP_000341066.1 hypothetical protein -
JW972_RS21420 (JW972_21360) 78804..79097 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
JW972_RS21425 (JW972_21365) 79102..80427 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
JW972_RS21430 (JW972_21370) 80488..80694 + 207 WP_000134171.1 hypothetical protein -
JW972_RS21435 (JW972_21375) 80796..81206 + 411 WP_000985911.1 hypothetical protein -
JW972_RS21440 (JW972_21380) 81219..82034 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
JW972_RS21445 (JW972_21385) 82288..82713 + 426 WP_001043843.1 hypothetical protein -
JW972_RS21450 (JW972_21390) 83261..83569 + 309 WP_001224687.1 hypothetical protein -
JW972_RS21455 83585..83923 + 339 WP_223678460.1 hypothetical protein -
JW972_RS21460 83983..84441 + 459 WP_072206020.1 HNH endonuclease signature motif containing protein -
JW972_RS21465 84503..84823 + 321 WP_223678484.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-1.1 / tet(M) / fosA3 / blaCTX-M-137 / aac(3)-IVa / aph(4)-Ia / sul2 / floR / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / dfrA12 / sul1 - 1..255882 255882


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T194546 WP_000088046.1 NZ_CP070874:79102-80427 [Escherichia fergusonii]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T194546 NZ_CP093548:c4608526-4608308 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT194546 WP_024136327.1 NZ_CP070874:78804-79097 [Escherichia fergusonii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT194546 NZ_CP093548:c4608926-4608552 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References