Detailed information of TA system TA194531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1795096..1795618
• Replicon: chromosome
• Accession: NZ_CP070873
• Organism: Escherichia fergusonii strain EF91

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JW972_RS08590
• Protein ID: WP_180491929.1
• Coordinates: 1795328..1795618 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SC69
• Locus tag: JW972_RS08585
• Protein ID: WP_000212715.1
• Coordinates: 1795096..1795338 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW972_RS08565 (1791013)	1791013..1792386	+	1374	WP_223666568.1	UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase	-
JW972_RS08570 (1792469)	1792469..1793020	-	552	WP_000166270.1	ribosome biogenesis factor YjgA	-
JW972_RS08575 (1793114)	1793114..1794466	+	1353	WP_001162171.1	metalloprotease PmbA	-
JW972_RS08580 (1794519)	1794519..1794905	+	387	WP_001232246.1	cytochrome b562	-
JW972_RS08585 (1795096)	1795096..1795338	+	243	WP_000212715.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
JW972_RS08590 (1795328)	1795328..1795618	+	291	WP_180491929.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JW972_RS08595 (1795619)	1795619..1796083	-	465	WP_001106232.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
JW972_RS08600 (1796268)	1796268..1798406	-	2139	WP_000187805.1	anaerobic ribonucleoside-triphosphate reductase	-
JW972_RS08605 (1798800)	1798800..1800455	-	1656	WP_223666567.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-72)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11350.29 Da
• Isoelectric Point: 10.3889

>T194531 WP_180491929.1 NZ_CP070873:1795328-1795618 [Escherichia fergusonii]
MNYELAFDPRALKEWQKLGVKVREQFKKKLGDVLKRPRNPSAKLRDLPDCYKIKLRTLGYRRVYQVNDKELIVLVIAIGK
RENSAVYEDATKRLDE

Nucleotide

• Length: 291 bp

>T194531 NZ_CP093548:c2302816-2302409 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9125.56 Da
• Isoelectric Point: 5.1369

>AT194531 WP_000212715.1 NZ_CP070873:1795096-1795338 [Escherichia fergusonii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Nucleotide

• Length: 243 bp

>AT194531 NZ_CP093548:c2303063-2302797 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGAATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAGCTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9H4B3