Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 294839..295493 Replicon chromosome
Accession NZ_CP070873
Organism Escherichia fergusonii strain EF91

Toxin (Protein)


Gene name cptA Uniprot ID F0JPE9
Locus tag JW972_RS01380 Protein ID WP_000244774.1
Coordinates 294839..295246 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JW972_RS01385 Protein ID WP_000354046.1
Coordinates 295227..295493 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW972_RS01360 (290796) 290796..292529 - 1734 WP_000813234.1 single-stranded-DNA-specific exonuclease RecJ -
JW972_RS01365 (292535) 292535..293245 - 711 WP_104917537.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JW972_RS01370 (293270) 293270..294166 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JW972_RS01375 (294278) 294278..294799 + 522 WP_001055865.1 flavodoxin FldB -
JW972_RS01380 (294839) 294839..295246 - 408 WP_000244774.1 protein YgfX Toxin
JW972_RS01385 (295227) 295227..295493 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JW972_RS01390 (295745) 295745..296725 + 981 WP_223666613.1 tRNA-modifying protein YgfZ -
JW972_RS01395 (296802) 296802..297461 - 660 WP_000250283.1 hemolysin III family protein -
JW972_RS01400 (297625) 297625..297936 - 312 WP_104917535.1 N(4)-acetylcytidine aminohydrolase -
JW972_RS01405 (297981) 297981..299414 + 1434 WP_148047390.1 6-phospho-beta-glucosidase BglA -
JW972_RS01410 (299462) 299462..300355 - 894 WP_000819362.1 transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16078.05 Da        Isoelectric Point: 11.2511

>T194524 WP_000244774.1 NZ_CP070873:c295246-294839 [Escherichia fergusonii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T194524 NZ_CP093548:c821745-821458 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT194524 WP_000354046.1 NZ_CP070873:c295493-295227 [Escherichia fergusonii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT194524 NZ_CP093548:c821984-821745 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L6ZX35

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References