Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 14448..15049 Replicon plasmid pGR0128-1842
Accession NZ_CP070850
Organism Lactiplantibacillus plantarum strain GR0128

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag JWR92_RS15575 Protein ID WP_001748110.1
Coordinates 14705..15049 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag JWR92_RS15570 Protein ID WP_001748109.1
Coordinates 14448..14711 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JWR92_RS15545 9720..10223 + 504 WP_046811094.1 DUF536 domain-containing protein -
JWR92_RS15550 10937..11314 + 378 WP_052748124.1 YxeA family protein -
JWR92_RS15555 12176..12960 - 785 Protein_14 IS5 family transposase -
JWR92_RS15560 13207..13401 - 195 WP_046811110.1 hypothetical protein -
JWR92_RS15565 13775..14362 - 588 WP_046811109.1 site-specific integrase -
JWR92_RS15570 14448..14711 + 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JWR92_RS15575 14705..15049 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JWR92_RS15580 15619..16299 - 681 WP_046811108.1 ABC transporter ATP-binding protein -
JWR92_RS15585 16304..17356 - 1053 WP_046811107.1 ABC transporter permease -
JWR92_RS15590 17482..17703 + 222 WP_046811106.1 transposase -
JWR92_RS15595 18034..18312 + 279 WP_003625288.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
JWR92_RS15600 18302..18604 + 303 WP_005917927.1 type II toxin-antitoxin system YafQ family toxin -
JWR92_RS15605 18727..19137 - 411 WP_046811105.1 hypothetical protein -
JWR92_RS15610 19253..19660 - 408 WP_046811104.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..31995 31995


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T194517 WP_001748110.1 NZ_CP070850:14705-15049 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T194517 NZ_CP093546:519275-519625 [Bacillus sp. K1]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT194517 WP_001748109.1 NZ_CP070850:14448-14711 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT194517 NZ_CP093546:518989-519270 [Bacillus sp. K1]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References