Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 508842..509478 | Replicon | chromosome |
| Accession | NZ_CP070844 | ||
| Organism | Bacillus subtilis subsp. natto strain BN0 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | JOW61_RS02615 | Protein ID | WP_003156187.1 |
| Coordinates | 509128..509478 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G4NU32 |
| Locus tag | JOW61_RS02610 | Protein ID | WP_003225183.1 |
| Coordinates | 508842..509123 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JOW61_RS02590 (JOW61_02570) | 505201..505800 | - | 600 | WP_014478896.1 | rhomboid family intramembrane serine protease | - |
| JOW61_RS02595 (JOW61_02575) | 505895..506260 | + | 366 | WP_014478897.1 | holo-ACP synthase | - |
| JOW61_RS02600 (JOW61_02580) | 506426..507442 | + | 1017 | WP_003234282.1 | outer membrane lipoprotein carrier protein LolA | - |
| JOW61_RS02605 (JOW61_02585) | 507557..508726 | + | 1170 | WP_014478898.1 | alanine racemase | - |
| JOW61_RS02610 (JOW61_02590) | 508842..509123 | + | 282 | WP_003225183.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| JOW61_RS02615 (JOW61_02595) | 509128..509478 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| JOW61_RS02620 (JOW61_02600) | 509594..510418 | + | 825 | WP_009966610.1 | RsbT co-antagonist protein RsbRA | - |
| JOW61_RS02625 (JOW61_02605) | 510423..510788 | + | 366 | WP_003225190.1 | RsbT antagonist protein RsbS | - |
| JOW61_RS02630 (JOW61_02610) | 510792..511193 | + | 402 | WP_014478899.1 | serine/threonine-protein kinase RsbT | - |
| JOW61_RS02635 (JOW61_02615) | 511205..512212 | + | 1008 | WP_014478900.1 | phosphoserine phosphatase RsbU | - |
| JOW61_RS02640 (JOW61_02620) | 512281..512610 | + | 330 | WP_003234298.1 | anti-sigma factor antagonist RsbV | - |
| JOW61_RS02645 (JOW61_02625) | 512607..513089 | + | 483 | WP_003234299.1 | anti-sigma B factor RsbW | - |
| JOW61_RS02650 (JOW61_02630) | 513055..513843 | + | 789 | WP_003246715.1 | RNA polymerase sigma factor SigB | - |
| JOW61_RS02655 (JOW61_02635) | 513843..514442 | + | 600 | WP_003246608.1 | phosphoserine phosphatase RsbX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T194515 WP_003156187.1 NZ_CP070844:509128-509478 [Bacillus subtilis subsp. natto]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T194515 NZ_CP093539:1133649-1134044 [Staphylococcus hominis]
ATGAAACAGATTGTATATTTAACTGAAAAACAAGTAATTGCCATTAACGTAAAAGCAATTCAAAATTTCTCACCGAAAGA
ACAAGTTGGTGTAAAAGTTCCAGAAGTTCTTAACGCTACCATCGAAGGAGTTAAACAATCATTCGGTGGAGTTGAACTAT
ATGAAACAATTGAGAGAAAAGCAGCTTTTATATATAGAAATATAGCTCAAAAGCATGCATTCCATAATGCGAATAAAAGA
ACAGCTTTTACATCTATGGTAATATTCTTGAAGTTAAATAGTATTAATTTTGAATGTACTCAAGATGAGGCGGTTCAGTT
TACTTTAAGAGTAGTAAATGACAAAACTCTTACTCTTGAAGAAATTGAAGCATGGATCAAAGGGCATTGTAAATAA
ATGAAACAGATTGTATATTTAACTGAAAAACAAGTAATTGCCATTAACGTAAAAGCAATTCAAAATTTCTCACCGAAAGA
ACAAGTTGGTGTAAAAGTTCCAGAAGTTCTTAACGCTACCATCGAAGGAGTTAAACAATCATTCGGTGGAGTTGAACTAT
ATGAAACAATTGAGAGAAAAGCAGCTTTTATATATAGAAATATAGCTCAAAAGCATGCATTCCATAATGCGAATAAAAGA
ACAGCTTTTACATCTATGGTAATATTCTTGAAGTTAAATAGTATTAATTTTGAATGTACTCAAGATGAGGCGGTTCAGTT
TACTTTAAGAGTAGTAAATGACAAAACTCTTACTCTTGAAGAAATTGAAGCATGGATCAAAGGGCATTGTAAATAA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10546.92 Da Isoelectric Point: 6.4708
>AT194515 WP_003225183.1 NZ_CP070844:508842-509123 [Bacillus subtilis subsp. natto]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT194515 NZ_CP093539:1133419-1133649 [Staphylococcus hominis]
ATGGTTACTACAAGAAAATTAAGACGAGCTGGTAATAGCTCAGTCGTTTCTGTGCCAACAGAAGTTATCGAAGCACTCGG
AGTCACAAATGGTGACAATCTTAGATTTAATGTCAAAAATAACCAAGTAACCATTGAAAAGGAGGTAAGTGAAGACGAAG
AGTTTTACAGAATGTTGGATGAGACATTTGCTGAGTACGACCAAGCTCTGAAAAGGATGGTCGAAGTGTAA
ATGGTTACTACAAGAAAATTAAGACGAGCTGGTAATAGCTCAGTCGTTTCTGTGCCAACAGAAGTTATCGAAGCACTCGG
AGTCACAAATGGTGACAATCTTAGATTTAATGTCAAAAATAACCAAGTAACCATTGAAAAGGAGGTAAGTGAAGACGAAG
AGTTTTACAGAATGTTGGATGAGACATTTGCTGAGTACGACCAAGCTCTGAAAAGGATGGTCGAAGTGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|